[CIP2A与OPN在膀胱癌中的表达及相关性]

[Expression and correlation of CIP2A and OPN in bladder cancer].

作者信息

Ma Tianjia, Xiao Pan, Xu Yang, Liu Yuqiang, Li Yutang, Shi Peikun, Zhou Yabin

机构信息

Department of Urology, Second Hospital of Shandong University, Shandong University Urologic Institute, Jinan 250033, China.

Department of Pathogen Biology, Shandong University, Jinan 250012, China. Email:

出版信息

Zhonghua Yi Xue Za Zhi. 2014 Oct 21;94(38):3005-7.

PMID:25547704

Abstract

OBJECTIVE

To explore the expressions of cancerous inhibitor of protein phosphatase 2A (CIP2A) and osteopontin (OPN) and evaluate their roles in bladder cancer.

METHODS

RNA was isolated from 38 cases of patients with bladder cancer and 12 cases of normal bladder tissue by TRIzol method from May 2010 to December 2012. And reverse transcription (RT)-PCR was used to detect the expressions of CIP2A and OPN. The expression levels of CIP2A and OPN in 99 cases of patients with bladder cancer and 12 cases of normal tissue were detected by immunohistochemical staining.

RESULTS

The positive expression rate of CIP2A mRNA and OPN mRNA were 76.32% (29/38) and 92.11% (35/38) in bladder cancer while there was no expression in normal tissue (both P < 0.05). The positive rates of CIP2A and OPN protein were 63.64% (63/99)and 84.85% (84/99)in cases of bladder cancer tissues while CIP2A was not detected in normal tissues. The positive expression rate of OPN in normal tissues was 2/12 (both P < 0.05). The CIP2A and OPN proteins were both expressed in 58/99 cases of bladder cancer tissues while neither of them was expressed in 13 cases. In 8 cases, CIP2A was expressed while OPN was not. In another 20 cases, OPN was expressed while CIP2A was not (r = 0.300, P < 0.05).

CONCLUSIONS

The expression levels of CIP2A and OPN in tissue of bladder cancer are higher than those of normal controls. And CIP2A and OPN may be used as indicators of biological behaviors and serve as new molecular diagnostic markers for bladder cancer.

摘要

目的

探讨蛋白磷酸酶2A癌性抑制剂（CIP2A）和骨桥蛋白（OPN）的表达情况，并评估它们在膀胱癌中的作用。

方法

采用TRIzol法从2010年5月至2012年12月期间的38例膀胱癌患者及12例正常膀胱组织中提取RNA。采用逆转录（RT）-PCR检测CIP2A和OPN的表达。采用免疫组织化学染色法检测99例膀胱癌患者及12例正常组织中CIP2A和OPN的表达水平。

结果

膀胱癌组织中CIP2A mRNA和OPN mRNA的阳性表达率分别为76.32%（29/38）和92.11%（35/38），而正常组织中均无表达（均P<0.05）。膀胱癌组织中CIP2A和OPN蛋白的阳性率分别为63.64%（63/99）和84.85%（84/99），正常组织中未检测到CIP2A，正常组织中OPN的阳性表达率为2/12（均P<0.05）。99例膀胱癌组织中有58例同时表达CIP2A和OPN蛋白，13例均不表达。8例仅表达CIP2A而不表达OPN。另外20例仅表达OPN而不表达CIP2A（r=0.300，P<0.05）。