脑桥被盖背外侧核或脑桥脚被盖核中胆碱能神经元的光遗传学激活可诱导快速眼动睡眠。
Optogenetic activation of cholinergic neurons in the PPT or LDT induces REM sleep.
作者信息
Van Dort Christa J, Zachs Daniel P, Kenny Jonathan D, Zheng Shu, Goldblum Rebecca R, Gelwan Noah A, Ramos Daniel M, Nolan Michael A, Wang Karen, Weng Feng-Ju, Lin Yingxi, Wilson Matthew A, Brown Emery N
机构信息
Department of Anesthesia, Critical Care, and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114; and Department of Brain and Cognitive Sciences, Picower Institute for Learning and Memory,
Department of Anesthesia, Critical Care, and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114; and Department of Brain and Cognitive Sciences, Picower Institute for Learning and Memory.
出版信息
Proc Natl Acad Sci U S A. 2015 Jan 13;112(2):584-9. doi: 10.1073/pnas.1423136112. Epub 2014 Dec 29.
Abstract
Rapid eye movement (REM) sleep is an important component of the natural sleep/wake cycle, yet the mechanisms that regulate REM sleep remain incompletely understood. Cholinergic neurons in the mesopontine tegmentum have been implicated in REM sleep regulation, but lesions of this area have had varying effects on REM sleep. Therefore, this study aimed to clarify the role of cholinergic neurons in the pedunculopontine tegmentum (PPT) and laterodorsal tegmentum (LDT) in REM sleep generation. Selective optogenetic activation of cholinergic neurons in the PPT or LDT during non-REM (NREM) sleep increased the number of REM sleep episodes and did not change REM sleep episode duration. Activation of cholinergic neurons in the PPT or LDT during NREM sleep was sufficient to induce REM sleep.
摘要
快速眼动（REM）睡眠是自然睡眠/觉醒周期的重要组成部分，但调节REM睡眠的机制仍未完全明确。脑桥中脑被盖的胆碱能神经元与REM睡眠调节有关，但该区域的损伤对REM睡眠产生了不同的影响。因此，本研究旨在阐明脚桥被盖（PPT）和外侧背盖（LDT）中的胆碱能神经元在REM睡眠产生中的作用。在非快速眼动（NREM）睡眠期间，对PPT或LDT中的胆碱能神经元进行选择性光遗传学激活，可增加REM睡眠发作次数，且不改变REM睡眠发作持续时间。在NREM睡眠期间激活PPT或LDT中的胆碱能神经元足以诱导REM睡眠。
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