Kempisty Bartosz, Walczak Rafał, Antosik Paweł, Sniadek Patrycja, Rybska Marta, Piotrowska Hanna, Bukowska Dorota, Dziuban Jan, Nowicki Michał, Jaśkowski Jędrzej M, Zabel Maciej, Brüssow Klaus-Peter
Department of Histology and Embryology, Poznan University of Medical Sciences, 6 Swiecickiego Street, 60-781 Poznan, Poland ; Department of Anatomy, Poznan University of Medical Science, 6 Swiecickiego Street, 60-781 Poznan, Poland.
Department of Microengineering and Photovoltaics, Wroclaw University of Technology, 11/17 Janiszewskiego Street, 50-372 Wroclaw, Poland.
Biomed Res Int. 2014;2014:467063. doi: 10.1155/2014/467063. Epub 2014 Jun 9.
Since microfollicular environment and the size of the follicle are important markers influencing oocyte quality, the aim of this study is to present the spectral characterization of oocytes isolated from follicles of various sizes using lab-on-chip (LOC) technology and to demonstrate how follicle size may affect oocyte quality. Porcine oocytes (each, n = 100) recovered from follicles of different sizes, for example, from large (>5 mm), medium (3-5 mm), and small (<3 mm), were analyzed after preceding in vitro maturation (IVM). The LOC analysis was performed using a silicon-glass sandwich with two glass optical fibers positioned "face-to-face." Oocytes collected from follicles of different size classes revealed specific and distinguishable spectral characteristics. The absorbance spectra (microspectrometric specificity) for oocytes isolated from large, medium, and small follicles differ significantly (P < 0.05) and the absorbance wavelengths were between 626 and 628 nm, between 618 and 620 nm, and less than 618 nm, respectively. The present study offers a parametric and objective method of porcine oocyte assessment. However, up to now this study has been used to evidence spectral markers associated with follicular size in pigs, only. Further investigations with functional-biological assays and comparing LOC analyses with fertilization and pregnancy success and the outcome of healthy offspring must be performed.
由于微卵泡环境和卵泡大小是影响卵母细胞质量的重要指标，本研究旨在利用芯片实验室（LOC）技术呈现从不同大小卵泡中分离出的卵母细胞的光谱特征，并证明卵泡大小如何影响卵母细胞质量。对从不同大小卵泡（例如，大卵泡（>5毫米）、中卵泡（3-5毫米）和小卵泡（<3毫米））中回收的猪卵母细胞（每组n = 100）进行体外成熟（IVM）预处理后进行分析。LOC分析使用硅-玻璃夹层，其中两根玻璃光纤“面对面”放置。从不同大小卵泡类别中收集的卵母细胞显示出特定且可区分的光谱特征。从大、中、小卵泡中分离出的卵母细胞的吸收光谱（显微光谱特异性）差异显著（P < 0.05），吸收波长分别在626至628纳米之间、618至620纳米之间以及小于618纳米。本研究提供了一种评估猪卵母细胞的参数化和客观方法。然而，到目前为止，这项研究仅用于证明与猪卵泡大小相关的光谱标记。必须进一步进行功能生物学测定，并将LOC分析与受精、妊娠成功率以及健康后代的结果进行比较。
