利用P1P4 - 双 -（5'，5'''-腺苷）四磷酸的各种亚甲基类似物对某些特定的Ap4A降解酶进行的研究。
Studies on some specific Ap4A-degrading enzymes with the use of various methylene analogues of P1P4-bis-(5',5'''-adenosyl) tetraphosphate.
作者信息
Guranowski A, Starzyńska E, Taylor G E, Blackburn G M
机构信息
Katedra Biochemii, Akademia Rolnicza, Poznań, Poland.
出版信息
Biochem J. 1989 Aug 15;262(1):241-4. doi: 10.1042/bj2620241.
Abstract
Six new methylenephosphonate analogues of P1P4-bis-(5',5'''-adenosyl) tetraphosphate, Ap4A, having P2-P3 carbon bridges CF2, CCl2 and CH2CH2 or P1-P2 and P3-P4 carbon bridges CF2, CCl2 and CH2CH2 in the tetraphosphate chain, were examined as substrates or inhibitors for two specific Ap4A-degrading enzymes: (asymmetrical) Ap4A hydrolase (EC 3.6.1.17) from yellow-lupin seeds and (symmetrical) Ap4A hydrolase (EC 3.6.1.41) from Escherichia coli. All analogues in which the central oxygen atom was replaced by a stable carbon bridge were hydrolysed by the asymmetrical hydrolase (CF2 greater than CCl2 greater than O greater than CHBr greater than CH2 greater than CH2CH2). As expected, these analogues were not hydrolysed by the symmetrical hydrolase, which was also unable to act on analogues having P1-P2 and P3-P4 carbon bridges.
摘要
研究了六种新的P1P4 - 双 -（5'，5'''-腺苷）四磷酸（Ap4A）的亚甲基膦酸酯类似物，它们在四磷酸链中具有P2 - P3碳桥CF2、CCl2和CH2CH2或P1 - P2和P3 - P4碳桥CF2、CCl2和CH2CH2，作为两种特定Ap4A降解酶的底物或抑制剂：来自黄羽扇豆种子的（不对称）Ap4A水解酶（EC 3.6.1.17）和来自大肠杆菌的（对称）Ap4A水解酶（EC 3.6.1.41）。所有中心氧原子被稳定碳桥取代的类似物都被不对称水解酶水解（CF2＞CCl2＞O＞CHBr＞CH2＞CH2CH2）。正如预期的那样，这些类似物不会被对称水解酶水解，对称水解酶也无法作用于具有P1 - P2和P3 - P4碳桥的类似物。
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