Li DongLiang, Wang Xiao, Liu Bang, Liu YuZheng, Zeng ZhiYu, Lu LingLing, Zheng ZhiYong, Li Bing, Zheng ZongFu
Department of Hepatobiliary Medicine, Fuzhou PLA General Hospital, Fuzhou Fujian, China.
Department of Pathology, Fuzhou PLA General Hospital, Fuzhou, Fujian, China.
PLoS One. 2014 Dec 30;9(12):e111741. doi: 10.1371/journal.pone.0111741. eCollection 2014.
To investigate injury pattern during intense exercises in hot and humid environment particularly on liver in a rat exertional heat stroke model.
We randomly divided 30 rats into a control group (CG), a normal temperature (25±2°C, 60%±5% humidity) exercise group (NTEG) and a high temperature and high humidity (35±2°C, 80%±10% humidity) exercising group (HTEG), each comprising 10 animals. The NTEG and HTEG rats were forced to run in a treadmill for 1 hour maximum at 20 rpm. We analyzed liver cells of all three groups with JC-1 dye and flow cytometry for apoptosis rates in addition to liver tissue 8 - hydroxy deoxyguanosine (8 - OhdG) and blood serum IL-6, tumor necrosis factor alpha (TNF-α), alanine aminotransferase ALT, aspartate amino transferase (AST), serum creatinine (CREA), blood urea nitrogen (BUN), lactate dehydrogenase (LDH), creatine phosphate kinase (CK) concentrations.
Compared with NTEG rats, beside reduced exercise tolerance (60±5 vs. 15±3 minutes) (p = 0.002) the 8-OhdG liver tissue concentrations were significantly higher (p = 0.040) in the HTEG rats. The HTEG developed more organ tissue damage and cellular fragmentations of liver cells. In both exercise groups TNF-α and IL-6 serum concentrations were enhanced significantly (p<0.001) being highest in the HTEG animals. Serum ALT, AST, LDH, CREA, BUN and CK concentrations were significantly enhance in both exercise groups.
In our exertional heat stroke rat model, we found tissue damage particularly in livers during exercises in hot and humid environment that was related to inflammation, oxidative stress and apoptosis.
在大鼠运动性中暑模型中，研究在炎热潮湿环境下剧烈运动期间的损伤模式，尤其是对肝脏的损伤模式。
我们将30只大鼠随机分为对照组（CG）、常温（25±2°C，湿度60%±5%）运动组（NTEG）和高温高湿（35±2°C，湿度80%±10%）运动组（HTEG），每组10只动物。NTEG组和HTEG组的大鼠被迫在跑步机上以20转/分钟的速度最多跑1小时。我们用JC-1染料和流式细胞术分析了所有三组的肝细胞凋亡率，此外还检测了肝组织8-羟基脱氧鸟苷（8-OhdG）以及血清白细胞介素-6（IL-6）、肿瘤坏死因子α（TNF-α）、丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、血清肌酐（CREA）、血尿素氮（BUN）、乳酸脱氢酶（LDH）、磷酸肌酸激酶（CK）的浓度。
与NTEG组大鼠相比，HTEG组大鼠除运动耐力降低（60±5分钟对15±3分钟）（p = 0.002）外，肝组织8-OhdG浓度显著更高（p = 0.040）。HTEG组出现了更多的器官组织损伤和肝细胞的细胞破碎。在两个运动组中，TNF-α和IL-6的血清浓度均显著升高（p<0.001），在HTEG组动物中最高。两个运动组的血清ALT、AST、LDH、CREA、BUN和CK浓度均显著升高。
在我们的运动性中暑大鼠模型中，我们发现在炎热潮湿环境下运动期间，特别是肝脏出现了组织损伤，这与炎症、氧化应激和细胞凋亡有关。
