Liu Sheng, Cimprich Jeffrey, Varisco Brian M
Division of Critical Care Medicine, Cincinnati Children's Hospital Medical Center.
Division of Critical Care Medicine, Cincinnati Children's Hospital Medical Center;
J Vis Exp. 2014 Dec 17(94):52294. doi: 10.3791/52294.
In humans, disrupted repair and remodeling of injured lung contributes to a host of acute and chronic lung disorders which may ultimately lead to disability or death. Injury-based animal models of lung repair and regeneration are limited by injury-specific responses making it difficult to differentiate changes related to the injury response and injury resolution from changes related to lung repair and lung regeneration. However, use of animal models to identify these repair and regeneration signaling pathways is critical to the development of new therapies aimed at improving pulmonary function following lung injury. The mouse pneumonectomy model utilizes compensatory lung growth to isolate those repair and regeneration signals in order to more clearly define mechanisms of alveolar re-septation. Here, we describe our technique for performing mouse pneumonectomy and sham pneumonectomy. This technique may be utilized in conjunction with lineage tracing or other transgenic mouse models to define molecular and cellular mechanism of lung repair and regeneration.
在人类中，受损肺脏的修复和重塑功能紊乱会引发一系列急慢性肺部疾病，最终可能导致残疾或死亡。基于损伤的肺修复和再生动物模型受限于损伤特异性反应，难以区分与损伤反应和损伤消退相关的变化以及与肺修复和肺再生相关的变化。然而，利用动物模型来识别这些修复和再生信号通路对于开发旨在改善肺损伤后肺功能的新疗法至关重要。小鼠肺切除术模型利用代偿性肺生长来分离那些修复和再生信号，以便更清晰地界定肺泡再分隔的机制。在此，我们描述了进行小鼠肺切除术和假手术的技术。该技术可与谱系追踪或其他转基因小鼠模型结合使用，以确定肺修复和再生的分子及细胞机制。
