Chen Jia Lin, Zhang Wei, Liu Ze Yu, Heng Boon Chin, Ouyang Hong Wei, Dai Xue Song
Mailbox #39 Center for Stem Cell and Tissue Engineering, School of Medicine, Zhejiang University, 866 Yu Hang Tang Road, Hangzhou, 310058, China.
Cell Tissue Res. 2015 May;360(2):195-207. doi: 10.1007/s00441-014-2077-4. Epub 2014 Dec 31.
Tendon injuries are commonly encountered in the clinic, disrupting the patient's normal work/life routine and damaging the career life of athletes. Currently, there is still no effective treatment for tendon injury. Tendon tissue engineering appears to be a promising route for tendon repair and regeneration. However, current strategies utilized in research are still far away from clinical applications due to unsuccessful cellular differentiation to tendon/tenocytes. In this review, we focus on the current physical strategies (mechanical stimulation and extracellular matrix topography) and evaluate their roles in precise and stepwise tendon differentiation. A systematic comprehension of normal tendon development process by structure, gene profile and physical microenvironment analysis is likely suggestive for stepwise tenocyte differentiation.
肌腱损伤在临床上很常见，会扰乱患者的正常工作/生活节奏，并损害运动员的职业生涯。目前，肌腱损伤仍没有有效的治疗方法。肌腱组织工程似乎是肌腱修复和再生的一条有前景的途径。然而，由于细胞向肌腱/腱细胞的分化未成功，目前研究中采用的策略仍远未达到临床应用阶段。在这篇综述中，我们聚焦于当前的物理策略（机械刺激和细胞外基质拓扑结构），并评估它们在精确且逐步的肌腱分化中的作用。通过结构、基因谱和物理微环境分析对正常肌腱发育过程进行系统理解，可能对腱细胞的逐步分化具有启示作用。
