Wagner Siegfried, Maibaum Denise, Pich Andreas, Nolte Ingo, Murua Escobar Hugo
Small Animal Clinic, University of Veterinary Medicine Hannover, Hannover, Germany.
Core Facility Proteomics, Hannover Medical School, Hannover, Germany.
Anticancer Res. 2015 Jan;35(1):145-8.
Canine prostate cancer (PC) is a highly aggressive malignancy. However, in contrast to man, neither standard screening strategies nor curative therapeutic options exist for the companion animal. A prostate-specific membrane antigen (PSMA) screening as molecular marker akin to human PC is currently not available for dogs as data on specific canine PSMA detection are contradictory.
To evaluate an antibody for specific canine PSMA detection by western blotting (WB), lysates of three canine prostatic cell lines (CT1258, DT08/40, DT08/46) were comparatively analyzed by WB and mass spectrometry (MS) to the human cell lines VCaP, LnCaP and PC-3.
MS analyses of the detected canine proteins confirmed cross reactivity of the antibody clone YPSMA-1 with canine PSMA.
The MS analyses of the extracted canine protein bands proved that the YPSMA-1 clone is as well specific for canine PSMA in WB and, thus, represents a reliable tool for comparative PSMA studies.
犬前列腺癌（PC）是一种侵袭性很强的恶性肿瘤。然而，与人类不同的是，伴侣动物既没有标准的筛查策略，也没有治愈性的治疗选择。目前，由于关于犬特异性前列腺特异性膜抗原（PSMA）检测的数据相互矛盾，类似于人类PC的作为分子标志物的PSMA筛查在犬中尚不可用。
为了通过蛋白质印迹法（WB）评估一种用于特异性检测犬PSMA的抗体，对三种犬前列腺细胞系（CT1258、DT08/40、DT08/46）的裂解物与人类细胞系VCaP、LnCaP和PC-3进行了WB和质谱（MS）比较分析。
对检测到的犬蛋白质进行的MS分析证实了抗体克隆YPSMA-1与犬PSMA的交叉反应性。
对提取的犬蛋白条带进行的MS分析证明，YPSMA-1克隆在WB中对犬PSMA也具有特异性，因此是进行PSMA比较研究的可靠工具。
