Heidtmann H H, Hofmann M, Jacob E, Erbil C, Havemann K, Schwartz-Albiez R
Department of Internal Medicine, Philipps University Marburg, Federal Republic of Germany.
Cancer Res. 1989 Dec 15;49(24 Pt 1):6960-5.
Several human lung tumor cell lines derived from large cell, squamous cell, and small cell carcinomas, as well as from mesotheliomas of the lung have been investigated for their gene expression and secretion of urokinase-type plasminogen activator (u-PA), tissue-type plasminogen activator (t-PA), and plasminogen activator inhibitors 1 and 2. All bronchogenic non-small cell carcinoma-derived cell lines studied could produce either plasminogen activators, their inhibitors, or both components, whereas in small cell lung carcinoma cell lines and cell lines derived from mesothelioma of the lung, no substantial amounts of any of these substances were synthesized. In detail, a large cell carcinoma-derived cell line, LCLC 97TM1, constitutively secreted large amounts of plasminogen activator. Northern blot analysis revealed RNA specific for u-PA and t-PA. Another large cell carcinoma-derived cell line, LCLC 103H, secreted smaller amounts of plasminogen activator and, additionally, plasminogen activator inhibitor. Specific mRNAs for u-PA and plasminogen activator inhibitors 1 and 2 were found in this cell line. In contrast, squamous cell carcinoma-derived cell lines secreted plasminogen activator only after treatment with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate; enhanced levels of u-PA, t-PA, and plasminogen activator inhibitor 1 mRNAs could then be demonstrated. The different expression of the plasminogen activator enzyme system distinguishes cell lines derived of non-small cell lung carcinoma from those of small cell lung carcinoma and may also reflect significant differences in the biological behavior of these tumor types.
已对几种源自大细胞癌、鳞状细胞癌、小细胞癌以及肺间皮瘤的人肺肿瘤细胞系进行了研究，分析其基因表达以及尿激酶型纤溶酶原激活物（u-PA）、组织型纤溶酶原激活物（t-PA）和纤溶酶原激活物抑制剂1和2的分泌情况。所有研究的源自支气管源性非小细胞癌的细胞系都能产生纤溶酶原激活物、其抑制剂或这两种成分，而在小细胞肺癌细胞系和源自肺间皮瘤的细胞系中，未合成任何这些物质的大量产物。具体而言，一种源自大细胞癌的细胞系LCLC 97TM1持续分泌大量纤溶酶原激活物。Northern印迹分析显示了u-PA和t-PA特异性的RNA。另一种源自大细胞癌的细胞系LCLC 103H分泌较少量的纤溶酶原激活物，此外还分泌纤溶酶原激活物抑制剂。在该细胞系中发现了u-PA和纤溶酶原激活物抑制剂1和2的特异性mRNA。相比之下，源自鳞状细胞癌的细胞系仅在用佛波酯12-O-十四酰佛波醇-13-乙酸酯处理后才分泌纤溶酶原激活物；随后可证明u-PA、t-PA和纤溶酶原激活物抑制剂1的mRNA水平升高。纤溶酶原激活物酶系统的不同表达将源自非小细胞肺癌的细胞系与小细胞肺癌的细胞系区分开来，也可能反映了这些肿瘤类型在生物学行为上的显著差异。
