Cha Yoon Jin, Kim Do Hee, Jung Woo Hee, Koo Ja Seung
Department of Pathology, Yonsei University College of Medicine, Severance Hospital Seodaemun-Gu, South Korea.
Int J Clin Exp Pathol. 2014 Oct 15;7(11):7824-33. eCollection 2014.
We aimed to evaluate the expression of sarcosine metabolism-related proteins according to site of metastatic breast cancer, and the clinical implications. Immunohistochemical staining for glycine N-methyltransferase (GNMT), sarcosine dehydrogenase (SARDH), and l-pipecolic acid oxidase (PIPOX) was performed on tissue microarrays from 162 metastatic breast cancer (bone metastases = 47, brain metastases = 39, liver metastases = 24, and lung metastases = 52). Sarcosine metabolism-related proteins showed variable expression with regard to metastatic sites. GNMT was expressed in brain and lung metastases, but not in bone and liver metastases (P = 0.016). In view of the sarcosine metabolic phenotype, high sarcosine and intermediate type were only found in the brain and lung metastases, and low sarcosine type was observed more frequently in bone and lung metastases (P = 0.047). By univariate analysis, PIPOX positivity was correlated with shorter overall survival (OS) (P = 0.031). In lung metastases, PIPOX positivity (P = 0.019) and stromal PIPOX positivity (P = 0.001) were associated with shorter OS. In conclusion, in metastatic breast cancer, sarcosine metabolism-related proteins are differently expressed according to the metastatic site. Expression of GNMT and high sarcosine type are predominantly observed in brain and lung metastases.
我们旨在根据转移性乳腺癌的部位评估肌氨酸代谢相关蛋白的表达情况及其临床意义。对162例转移性乳腺癌（骨转移47例、脑转移39例、肝转移24例、肺转移52例）的组织芯片进行甘氨酸N-甲基转移酶（GNMT）、肌氨酸脱氢酶（SARDH）和L-哌啶酸氧化酶（PIPOX）的免疫组化染色。肌氨酸代谢相关蛋白在不同转移部位表现出不同的表达。GNMT在脑转移和肺转移中表达，但在骨转移和肝转移中不表达（P = 0.016）。就肌氨酸代谢表型而言，高肌氨酸型和中间型仅在脑转移和肺转移中发现，低肌氨酸型在骨转移和肺转移中更常见（P = 0.047）。单因素分析显示，PIPOX阳性与总生存期（OS）较短相关（P = 0.031）。在肺转移中，PIPOX阳性（P = 0.019）和基质PIPOX阳性（P = 0.001）与较短的OS相关。总之，在转移性乳腺癌中，肌氨酸代谢相关蛋白根据转移部位的不同而有不同表达。GNMT的表达和高肌氨酸型主要见于脑转移和肺转移。
