Juvenal G J, Kleiman de Pisarev D L, Crenovich L, Pisarev M A
Acta Endocrinol (Copenh). 1978 Apr;87(4):776-85. doi: 10.1530/acta.0.0870776.
The present studies were performed in order to investigate the role of neurotransmitters, prostaglandins and glucose on [3H] uridine incorporation into total RNA in beef thyroid slices. Carbamylcholine strongly stimulated RNA labelling from [3H uridine; atropine abolished this effect. NaF, at 1 and 5 mM, progressively increased this parameter while norepinephrine caused a similar effect at 10(-3) but not at 10(-6) M. Phentolamine (1 mM) blocked the stimulatory action of TSH; propranolol and atropine did not. Glucose at concentrations between 4 and 24 mM caused a progressive increase in RNA labelling from [3H] uridine. This effect was inhibited by dinitrophenol. Prostaglandins (E1, A1, F1alpha and F2alpha) assayed in concentrations between 5 and 25 microgram/ml, with or without caffeine, had no effect on RNA labelling. Moreover, neither aspirin nor indomethacin inhibited TSH stimulation. Under similar experimental conditions, PGE1 did simulate PB125I formation.
进行本研究是为了探讨神经递质、前列腺素和葡萄糖对牛肉甲状腺切片中[3H]尿苷掺入总RNA的作用。氨甲酰胆碱强烈刺激[3H]尿苷的RNA标记；阿托品消除了这种作用。1 mM和5 mM的氟化钠使该参数逐渐增加，而10(-3) M的去甲肾上腺素产生类似作用，但10(-6) M时则无此作用。1 mM的酚妥拉明阻断促甲状腺激素的刺激作用；普萘洛尔和阿托品则无此作用。4 mM至24 mM浓度的葡萄糖使[3H]尿苷的RNA标记逐渐增加。这种作用被二硝基苯酚抑制。浓度在5至25微克/毫升之间的前列腺素（E1、A1、F1α和F2α），无论有无咖啡因，对RNA标记均无影响。此外，阿司匹林和吲哚美辛均不抑制促甲状腺激素的刺激作用。在相似的实验条件下，PGE1确实模拟了PB125I的形成。
