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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Department of Biochemistry, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.

Int J Biochem. 1989;21(10):1157-9. doi: 10.1016/0020-711x(89)90059-1.

The activity of cAMP phosphodiesterase (PDE) was studied in a 10,000 g particulate fraction prepared from rat brain. 2. Phospholipase C such as sphingomyelin choline phosphodiesterase (SMase), phosphatidylinositol phosphodiesterase (PIase) and phosphatidylcholine phosphohydrolase (PCase) were used to deplete phospholipid(s) from the particulate fraction and their effects on PDE activity were investigated. 3. Treatment with SMase or PIase did not affect PDE activity whereas treatment with PCase resulted in inhibition. 4. It was also found that the PCase used not only hydrolyzed phosphatidylcholine but also other phospholipids such as phosphatidylethanolamine, phosphatidylserine and sphingomyelin.

研究了从大鼠脑制备的10,000 g微粒组分中cAMP磷酸二酯酶（PDE）的活性。2. 使用磷脂酶C，如鞘磷脂胆碱磷酸二酯酶（SMase）、磷脂酰肌醇磷酸二酯酶（PIase）和磷脂酰胆碱磷酸水解酶（PCase）从微粒组分中消耗磷脂，并研究它们对PDE活性的影响。3. 用SMase或PIase处理不影响PDE活性，而用PCase处理则导致抑制。4. 还发现所用的PCase不仅水解磷脂酰胆碱，还水解其他磷脂，如磷脂酰乙醇胺、磷脂酰丝氨酸和鞘磷脂。

Department of Biochemistry, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.

Int J Biochem. 1989;21(10):1157-9. doi: 10.1016/0020-711x(89)90059-1.

The activity of cAMP phosphodiesterase (PDE) was studied in a 10,000 g particulate fraction prepared from rat brain. 2. Phospholipase C such as sphingomyelin choline phosphodiesterase (SMase), phosphatidylinositol phosphodiesterase (PIase) and phosphatidylcholine phosphohydrolase (PCase) were used to deplete phospholipid(s) from the particulate fraction and their effects on PDE activity were investigated. 3. Treatment with SMase or PIase did not affect PDE activity whereas treatment with PCase resulted in inhibition. 4. It was also found that the PCase used not only hydrolyzed phosphatidylcholine but also other phospholipids such as phosphatidylethanolamine, phosphatidylserine and sphingomyelin.

研究了从大鼠脑制备的10,000 g微粒组分中cAMP磷酸二酯酶（PDE）的活性。2. 使用磷脂酶C，如鞘磷脂胆碱磷酸二酯酶（SMase）、磷脂酰肌醇磷酸二酯酶（PIase）和磷脂酰胆碱磷酸水解酶（PCase）从微粒组分中消耗磷脂，并研究它们对PDE活性的影响。3. 用SMase或PIase处理不影响PDE活性，而用PCase处理则导致抑制。4. 还发现所用的PCase不仅水解磷脂酰胆碱，还水解其他磷脂，如磷脂酰乙醇胺、磷脂酰丝氨酸和鞘磷脂。