集胞藻PCC6803中衣康酸的光合生产
Photosynthetic production of itaconic acid in Synechocystis sp. PCC6803.
作者信息
Chin Taejun, Sano Mei, Takahashi Tetsuya, Ohara Hitomi, Aso Yuji
机构信息
Department of Biobased Materials Science, Kyoto Institute of Technology, 1 Hashigami-cho, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
Faculty of Education, Shimane University, 1060 Nishikawatsu-cho, Matsue, Shimane 690-8504, Japan.
出版信息
J Biotechnol. 2015 Feb 10;195:43-5. doi: 10.1016/j.jbiotec.2014.12.016. Epub 2014 Dec 30.
Here, we report the photosynthetic production of itaconic acid (IA), a promising building block, from carbon dioxide (CO₂) by Synechocystis sp. PCC6803. The engineered PCC6803 strain expressing cis-aconitate decarboxylase, the key enzyme in IA biosynthesis, produced 0.9 mg/L and 14.5 mg/L of IA at production rates of 42.8 μgL(-1)day(-1) and 919.0 μgL(-1)day(-1), under conditions of constant bubbling with air and 5% CO₂, respectively. This is the first report on the possibility of IA production from CO₂ via the photosynthetic process in cyanobacteria.
在此，我们报道了集胞藻PCC6803从二氧化碳（CO₂）光合生产衣康酸（IA）的过程，衣康酸是一种很有前景的基础原料。表达顺乌头酸脱羧酶（IA生物合成中的关键酶）的工程化PCC6803菌株，在分别以空气和5% CO₂持续鼓泡的条件下，IA的产量分别为0.9 mg/L和14.5 mg/L，生产速率分别为42.8 μg L⁻¹天⁻¹和919.0 μg L⁻¹天⁻¹。这是关于蓝细菌通过光合过程从CO₂生产IA可能性的首次报道。
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