亚致死浓度酸化水对白色念珠菌的抗真菌样作用。
Postantifungal-like effect of sublethal treatment of Candida albicans with acid-electrolyzed water.
机构信息
Tohoku University Graduate School of Dentistry, 4-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan.
Tohoku University Graduate School of Dentistry, 4-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan.
出版信息
Arch Oral Biol. 2015 Mar;60(3):479-87. doi: 10.1016/j.archoralbio.2014.12.008. Epub 2014 Dec 22.
OBJECTIVE
Acid-electrolyzed water (AEW) has been applied to the treatment of oral candidiasis. We evaluated the postantifungal effect (PAFE)-like activity of AEW against Candida albicans under sublethal conditions by exposing C. albicans to dilute AEW.
DESIGN
The growth of C. albicans after a short-term exposure to dilute AEW was evaluated in broth and on agar culture. The involvement of reactive oxygen species (ROS) in the PAFE was examined by flow cytometric analysis with hydroxyphenyl fluorescein (HPF) as a fluorescence probe.
RESULTS
The dilute AEW exerted PAFE-like activity against C. albicans. ROS were produced in the cells treated with AEW diluted 16 times or fewer. The increase in HPF fluorescence after treatment with dilute AEW was cancelled by dimethyl sulfoxide, a hydroxyl radical (OH) scavenger.
CONCLUSION
It would be expected that the ROS, especially OH, produced in the C. albicans cells treated with sublethal dilutions of AEW could exert PAFE-like activity against the fungal cells.
目的
酸化水（AEW）已被应用于口腔念珠菌病的治疗。我们通过使白色念珠菌暴露于稀释的 AEW 来评估亚致死条件下 AEW 对白色念珠菌的抗真菌后效应（PAFE）样活性。
设计
在肉汤和琼脂培养物中评估白色念珠菌在短期暴露于稀释的 AEW 后的生长情况。通过使用羟苯基荧光素（HPF）作为荧光探针的流式细胞术分析来检查活性氧物质（ROS）在 PAFE 中的作用。
结果
稀释的 AEW 对白色念珠菌表现出 PAFE 样活性。用稀释 16 倍或更少的 AEW 处理的细胞中产生了 ROS。用亚致死稀释的 AEW 处理后，HPF 荧光的增加被二甲基亚砜（OH 自由基清除剂）所消除。
结论
预计用亚致死稀释的 AEW 处理的白色念珠菌细胞中产生的 ROS，特别是 OH，可能对真菌细胞发挥 PAFE 样活性。
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