Singh Ajit, Suragani Madhuri, Ehtesham Nasreen Z, Krishna Amitabh
Reproductive Endocrinology Lab, Department of Zoology, Banaras Hindu University, Varanasi 221005, India.
Dr. Reddy's Institute of Life Sciences, Hyderabad University Campus, Gachibowly, Hyderabad 500046, India.
Steroids. 2015 Mar;95:17-23. doi: 10.1016/j.steroids.2014.12.018. Epub 2014 Dec 30.
The aim of the present study was to evaluate the expression and effect of resistin on ovarian activities of Scotophilus heathi. Immunohistochemical study showed marked variation in resistin immunostaining during different reproductive phases. Most intense immunostaining of resistin was noticed in thecal-interstitial cells in ovary during the period of delayed ovulation, the period of increased androgen synthesis and suppressed ovulation. The changes in ovarian resistin level also correlated positively with circulating leptin level and body white adipose tissue accumulation. The in vitro study showed that resistin alone preferentially stimulated progesterone synthesis, but with luteinizing hormone (LH) stimulated androgen secretion. Resistin alone dose-dependently increased expression of LH-receptor, steroidogenic acute regulatory protein and insulin receptor proteins in the ovary, whereas together with LH showed dose-dependent stimulatory effect on expression of androgen receptor and insulin receptor proteins in the ovary. In conclusion, during the period of fat accumulation increased ovarian resistin level may be responsible for increased androgen synthesis through insulin receptor mediated pathways in the ovary of S. heathi.
本研究的目的是评估抵抗素对印度假吸血蝠卵巢活动的表达及影响。免疫组织化学研究显示，在不同生殖阶段，抵抗素免疫染色存在显著差异。在排卵延迟、雄激素合成增加及排卵受抑制期间，卵巢的卵泡膜间质细胞中抵抗素免疫染色最为强烈。卵巢抵抗素水平的变化也与循环瘦素水平及体白色脂肪组织积累呈正相关。体外研究表明，单独的抵抗素优先刺激孕酮合成，但与促黄体生成素（LH）共同作用时则刺激雄激素分泌。单独的抵抗素剂量依赖性地增加卵巢中LH受体、类固醇生成急性调节蛋白和胰岛素受体蛋白的表达，而与LH共同作用时对卵巢中雄激素受体和胰岛素受体蛋白的表达呈剂量依赖性刺激作用。总之，在脂肪积累期间，卵巢抵抗素水平升高可能通过胰岛素受体介导的途径导致印度假吸血蝠卵巢雄激素合成增加。
