Lin Chien Y, Huang Jung Y, Lo Leu-Wei
Department of Photonics, Chiao Tung University, Hsinchu 300, Taiwan, ROC.
The T.K.P. Research Center for Photonics, Chiao Tung University, Hsinchu 300, Taiwan, ROC.
Biochim Biophys Acta. 2015 Mar;1848(3):886-93. doi: 10.1016/j.bbamem.2014.12.019. Epub 2014 Dec 30.
Epidermal growth factor receptor (EGFR/ErbB1) is a transmembrane protein that can drive cell growth and survival via the ligand-induced dimerization of receptors. Because dimerization is a common mechanism for signal transduction, it is important to improve our understanding of how the dimerization process and membrane structure regulate signal transduction. In this study, we examined the effect of lipid nanodomains on the dimerization process of EGFR molecules. We discovered that after ligand binding, EGFR molecules may move into lipid nanodomains. The lipid nanodomains surrounding two liganded EGFRs can merge during their correlated motion. The transition rates between different diffusion states of liganded EGFR molecules are regulated by the lipid domains. Our method successfully captures both the sensitivity of single-molecule processes and statistic accuracy of data analysis, providing insight into the connection between the mobile clustering process of receptors and the hierarchical structure of plasma membrane.
表皮生长因子受体（EGFR/ErbB1）是一种跨膜蛋白，它可通过配体诱导的受体二聚化来驱动细胞生长和存活。由于二聚化是信号转导的常见机制，因此增进我们对二聚化过程和膜结构如何调节信号转导的理解非常重要。在本研究中，我们研究了脂质纳米域对EGFR分子二聚化过程的影响。我们发现，在配体结合后，EGFR分子可能会进入脂质纳米域。围绕两个配体化EGFR的脂质纳米域在其相关运动过程中可能会合并。配体化EGFR分子不同扩散状态之间的转变速率受脂质域调节。我们的方法成功地捕捉了单分子过程的敏感性和数据分析的统计准确性，为深入了解受体的移动聚集过程与质膜分层结构之间的联系提供了线索。
