Chen Yang, Liu Zhong-Jing, Liu Jia, Liu Li-Kun, Zhang Er-Shao, Li Wei-Ling
Department of Biotechnology, Dalian Medical University, Dalian, Liaoning, China E-mail :
Asian Pac J Cancer Prev. 2014;15(23):10351-4. doi: 10.7314/apjcp.2014.15.23.10351.
Rosa Roxburghii Tratt is a promising wild fruit crop in Southwest China. Its extracts have been used as traditional Chinese medicine, which benefit immune responses and cure various health disorders. However, whether Rosa Roxburghii Tratt polysaccharides could inhibit metastasis and invasion of ovarian cancer cells remains unknown.
Effects of crude polysaccharides from Rosa Roxburghii Tratt on the viability of ovarian cancer A2780 cells were detected by MTT assay. Ovarian carcinoma cell migration and invasion after exposure to Rosa Roxburghii Tratt polysaccharides were quantified by wound healing and Transwell assays, respectively. Western blotting was applied to assess protein levels of MMP-9.
The results indicated that Rosa Roxburghii Tratt polysaccharides significantly reduced wound closure rate of A2780 cells, inhibited their migration and invasion, and suppressed the expression of MMP-9.
Our findings indicated that Rosa Roxburghii Tratt polysaccharides have potential for develop as anti-metastatic cancer drug preparations for ovarian cancer patients.
刺梨是中国西南地区一种很有前景的野生水果作物。其提取物已被用作中药，有益于免疫反应并治疗各种健康疾病。然而，刺梨多糖是否能抑制卵巢癌细胞的转移和侵袭仍不清楚。
采用MTT法检测刺梨粗多糖对卵巢癌A2780细胞活力的影响。分别通过伤口愈合实验和Transwell实验对暴露于刺梨多糖后的卵巢癌细胞迁移和侵袭情况进行定量分析。应用蛋白质免疫印迹法评估基质金属蛋白酶-9（MMP-9）的蛋白水平。
结果表明，刺梨多糖显著降低了A2780细胞的伤口愈合率，抑制了其迁移和侵袭，并抑制了MMP-9的表达。
我们的研究结果表明，刺梨多糖有潜力开发成为卵巢癌患者的抗转移癌症药物制剂。
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