拉脱维亚耐氨基糖苷类结核分枝杆菌分离株的基因型和表型特征
Genotypic and phenotypic characteristics of aminoglycoside-resistant Mycobacterium tuberculosis isolates in Latvia.
作者信息
Bauskenieks Matiss, Pole Ilva, Skenders Girts, Jansone Inta, Broka Lonija, Nodieva Anda, Ozere Iveta, Kalvisa Adrija, Ranka Renate, Baumanis Viesturs
机构信息
Latvian Biomedical Research and Study Centre, Ratsupites Street 1, Riga, LV-1067, Latvia.
Latvian Biomedical Research and Study Centre, Ratsupites Street 1, Riga, LV-1067, Latvia; Center of Tuberculosis and Lung Diseases, Riga East University Hospital, Stopinu p., Riga, LV-2118, Latvia.
出版信息
Diagn Microbiol Infect Dis. 2015 Mar;81(3):177-82. doi: 10.1016/j.diagmicrobio.2014.12.004. Epub 2014 Dec 15.
Mutations causing resistance to aminoglycosides, such as kanamycin (KAN), amikacin (AMK), and streptomycin, are not completely understood. In this study, polymorphisms of aminoglycoside resistance influencing genes such as rrs, eis, rpsL, and gidB in 41 drug-resistant and 17 pan-sensitive Mycobacterium tuberculosis clinical isolates in Latvia were analyzed. Mutation A1400G in rrs gene was detected in 92% isolates with high resistance level to KAN and diverse MIC level to AMK. Mutations in promoter region of eis were detected in 80% isolates with low-level MIC of KAN. The association of K43R mutation in rpsL gene, a mutation in the rrs gene at position 513, and various polymorphisms in gidB gene with distinct genetic lineages of M. tuberculosis was observed. The results of this study suggest that association of different controversial mutations of M. tuberculosis genes to the drug resistance phenotype should be done in respect to genetic lineages.
导致对氨基糖苷类抗生素（如卡那霉素（KAN）、阿米卡星（AMK）和链霉素）产生耐药性的突变尚未完全明确。在本研究中，对拉脱维亚41株耐药和17株全敏感结核分枝杆菌临床分离株中影响氨基糖苷类耐药性的基因（如rrs、eis、rpsL和gidB）的多态性进行了分析。在对KAN具有高耐药水平且对AMK有不同MIC水平的92%的分离株中检测到rrs基因中的A1400G突变。在KAN低水平MIC的80%的分离株中检测到eis启动子区域的突变。观察到rpsL基因中的K43R突变、rrs基因第513位的突变以及gidB基因中的各种多态性与结核分枝杆菌不同遗传谱系之间的关联。本研究结果表明，应针对遗传谱系来研究结核分枝杆菌基因不同的有争议突变与耐药表型之间的关联。
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