Pang Hui, Wan Kanglin, Wei Lin
Department of Immunology, Hebei Medical University, Shijiazhuang, Hebei, China.
Department of Immunology, Changzhi Medical College, Changzhi, Shanxi, China.
Infect Drug Resist. 2018 Apr 9;11:515-521. doi: 10.2147/IDR.S160899. eCollection 2018.
The relationships between fluoroquinolone and aminoglycoside resistance and single-nucleotide polymorphisms (SNPs) in , , and genes were investigated in 95 clinical isolates of from China.
Fluoroquinolone and aminoglycoside resistance were determined by the broth microdilution method. , , and were sequenced, SNPs were identified, and the corresponding amino acid mutations were recorded.
The isolates displayed high levels of ofloxacin (93.68%), ciprofloxacin (92.63%), and streptomycin (65.26%) resistance. Moxifloxacin (18.95%) and amikacin (2.11%) were highly active against the strains. Fluoroquinolone resistance involving and gene mutations was identified. For , the most frequent SNPs were T→C (71/95, 74.74%), followed by A→G (64/95, 67.37%) and T→C (62/95, 65.26%). The amino acid mutations occurred mainly at Gly2444Asp (GGT→GAT) (20/95, 21.05%), Ala2445Ser (GCC→TCC) (20/95, 21.05%), Ala2447Val (GCC→GTC) (20/95, 21.05%), Val2449Ile (GTC→ATC) (20/95, 21.05%), and Glu2450Gln (GAA→CAA) (20/95, 21.05%). Prominent SNPs in included A→C (69/95, 72.63%), C→T (51/95, 53.68%), and T→G (29/95, 30.53%), and their amino acid substitutions were Ile2160Val (ATT→GTT) (21/95, 22.11%), Ile2160Met (ATT→ATG) (20/95, 21.05%), and Ile2273Leu (ATC→CTC) (11/95, 11.58%). Among the strains with aminoglycoside resistance, SNPs in were identified mostly at position G→A (73/95, 76.84%). G→C (21/95, 22.11%) was commonly seen. The amino acid mutations primarily involved Ala1539985Thr (GCC→ACC) (19/95, 20.00%), His1539992Asp (CAC→GAC) (19/95, 20.00%), and Gln-1539983Glu (CAG→GAG) (18/95, 18.95%).
Our study provides valuable information that could be used for the future diagnosis and treatment of disease.
在中国的95株临床分离菌株中，研究氟喹诺酮类和氨基糖苷类耐药性与、和基因中的单核苷酸多态性（SNP）之间的关系。
采用肉汤微量稀释法测定氟喹诺酮类和氨基糖苷类耐药性。对、和进行测序，鉴定SNP，并记录相应的氨基酸突变。
这些分离菌株对氧氟沙星（93.68%）、环丙沙星（92.63%）和链霉素（65.26%）呈现出高水平耐药。莫西沙星（18.95%）和阿米卡星（2.11%）对这些菌株具有高活性。鉴定出涉及和基因突变的氟喹诺酮类耐药性。对于，最常见的SNP是T→C（71/95，74.74%），其次是A→G（64/95，67.37%）和T→C（62/95，65.26%）。氨基酸突变主要发生在Gly2444Asp（GGT→GAT）（20/95，21.05%）、Ala2445Ser（GCC→TCC）（20/95，21.05%）、Ala2447Val（GCC→GTC）（20/95，21.05%）、Val2449Ile（GTC→ATC）（20/95，21.05%）和Glu2450Gln（GAA→CAA）（20/95，21.05%）。中显著的SNP包括A→C（69/95，72.63%）、C→T（51/95，53.68%）和T→G（29/95，30.53%），其氨基酸替代为Ile2160Val（ATT→GTT）（21/95，22.11%）、Ile2160Met（ATT→ATG）（20/95，21.05%）和Ile2273Leu（ATC→CTC）（11/95，11.58%）。在具有氨基糖苷类耐药性的菌株中，中SNP大多在G→A位置（73/95，76.84%）被鉴定出。G→C（21/95，22.11%）也较为常见。氨基酸突变主要涉及Ala1539985Thr（GCC→ACC）（19/95，20.00%）、His1539992Asp（CAC→GAC）（19/95，20.00%）和Gln-1539983Glu（CAG→GAG）（18/95，18.95%）。
我们的研究提供了有价值的信息，可用于未来疾病的诊断和治疗。
