Asian-Australas J Anim Sci. 2015 Feb;28(2):231-8. doi: 10.5713/ajas.14.0039.
An experiment was conducted to increase the quality of rice bran by fermentation using Bacillus amyloliquefaciens and humic substances and its utilization as a feed ingredient for broiler chickens. The experiment was carried out in two steps. First, the fermentation process was done using a completely randomized design in factorial with 16 treatments: i) Dosage of B. amyloliquefaciens (2.10(8) cfu/g), 10 and 20 g/kg; ii) Graded levels of humic substances, 0, 100, 200, and 400 ppm; iii) Length of fermentation, three and five days. The results showed that the fermentation significantly (p<0.05) reduced crude fiber content. The recommended conditions for fermentation of rice bran: 20 g/kg dosage of inoculums B. amyloliquefaciens, 100 ppm level of humic substances and three days fermentation period. The second step was a feeding trial to evaluate the fermented rice bran (FRB) as a feed ingredient for broiler chickens. Three hundred and seventy-five one-day-old broiler chicks were randomly assigned into five treatment diets. Arrangement of the diets as follows: 0%, 5%, 10%, 15%, and 20% level of FRB and the diets formulation based on equal amounts of energy and protein. The results showed that 15% inclusion of FRB in the diet provided the best bodyweight gain and feed conversion ratio (FCR) values. In conclusion, the nutrient content of rice bran improved after fermentation and the utilization of FRB as a feed ingredient for broiler chickens could be included up to 15% of the broiler diet.
采用解淀粉芽孢杆菌和腐植酸对米糠进行发酵以提高其质量,并将其作为肉鸡饲料原料进行利用。试验分两步进行。首先,采用完全随机设计,在两因素析因试验中进行发酵处理,共 16 个处理:i)解淀粉芽孢杆菌(2.10(8)cfu/g)添加量,10 和 20 g/kg;ii)腐植酸分级水平,0、100、200 和 400 ppm;iii)发酵时间,3 和 5 天。结果表明,发酵显著(p<0.05)降低了粗纤维含量。米糠发酵的推荐条件为:接种物解淀粉芽孢杆菌添加量 20 g/kg、腐植酸水平 100 ppm 和发酵 3 天。第二步是评价发酵米糠(FRB)作为肉鸡饲料原料的饲养试验。将 375 只 1 日龄肉鸡随机分配到 5 种处理日粮中。日粮安排如下:0%、5%、10%、15%和 20%的 FRB 水平,日粮配方基于等量的能量和蛋白质。结果表明,日粮中添加 15%的 FRB 可获得最佳的体重增加和饲料转化率(FCR)值。总之,米糠经发酵后其营养成分得到改善,FRB 作为肉鸡饲料原料的利用率可达到肉鸡日粮的 15%。