Wen Liang, Xu Jun, Zhan Tianxiang, Wang Hao, Huang Xin, Liu Wenchao, Yang Xiaofeng, Zhan Renya
Department of Neurosurgery, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China.
Neural Regen Res. 2014 Nov 1;9(21):1902-6. doi: 10.4103/1673-5374.145358.
The accumulation of myelin debris may be a major contributor to the inflammatory response after diffuse axonal injury. In this study, we examined the accumulation and clearance of myelin debris in a rat model of diffuse axonal injury. Oil Red O staining was performed on sections from the cerebral cortex, hippocampus and brain stem to identify the myelin debris. Seven days after diffuse axonal injury, many Oil Red O-stained particles were observed in the cerebral cortex, hippocampus and brain stem. In the cerebral cortex and hippocampus, the amount of myelin debris peaked at 14 days after injury, and decreased significantly at 28 days. In the brain stem, the amount of myelin debris peaked at 7 days after injury, and decreased significantly at 14 and 28 days. In the cortex and hippocampus, some myelin debris could still be observed at 28 days after diffuse axonal injury. Our findings suggest that myelin debris may persist in the rat central nervous system after diffuse axonal injury, which would hinder recovery.
髓磷脂碎片的积累可能是弥漫性轴索损伤后炎症反应的主要促成因素。在本研究中，我们在弥漫性轴索损伤大鼠模型中检测了髓磷脂碎片的积累和清除情况。对大脑皮质、海马体和脑干的切片进行油红O染色以识别髓磷脂碎片。弥漫性轴索损伤7天后，在大脑皮质、海马体和脑干中观察到许多油红O染色颗粒。在大脑皮质和海马体中，髓磷脂碎片的量在损伤后14天达到峰值，并在28天时显著减少。在脑干中，髓磷脂碎片的量在损伤后7天达到峰值，并在14天和28天时显著减少。在弥漫性轴索损伤28天后，在皮质和海马体中仍可观察到一些髓磷脂碎片。我们的研究结果表明，弥漫性轴索损伤后髓磷脂碎片可能会在大鼠中枢神经系统中持续存在，这将阻碍恢复。
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