利用工程化甲苯单加氧酶将乙烯生物催化转化为环氧乙烷。
Biocatalytic conversion of ethylene to ethylene oxide using an engineered toluene monooxygenase.
作者信息
Carlin D A, Bertolani S J, Siegel J B
机构信息
Biophysics Graduate Group, University of California Davis, Davis, California, USA.
出版信息
Chem Commun (Camb). 2015 Feb 11;51(12):2283-5. doi: 10.1039/c4cc08802f.
PMID:25558486
Abstract
Mutants of toluene o-xylene monooxygenase are demonstrated to oxidize ethylene to ethylene oxide in vivo at yields of >99%. The best mutant increases ethylene oxidation activity by >5500-fold relative to the native enzyme. This is the first report of a recombinant enzyme capable of carrying out this industrially significant chemical conversion.
摘要
已证明甲苯邻二甲苯单加氧酶的突变体能够在体内将乙烯氧化为环氧乙烷，产率>99%。最佳突变体相对于天然酶将乙烯氧化活性提高了>5500倍。这是关于一种能够进行这种具有工业重要性的化学转化的重组酶的首次报道。
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