Sinthusith Nutpornnapat, Terada Akihiko, Hahn Martha, Noophan Pongsak Lek, Munakata-Marr Junko, Figueroa Linda A
a Department of Environmental Engineering, Faculty of Engineering , Kasetsart University , Bangkok , Thailand.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2015;50(2):169-75. doi: 10.1080/10934529.2014.975535.
In this study, the abundance and sequences of the amoA gene in ammonia oxidizing bacteria (AOB) and ammonia oxidizing archaea (AOA) were defined in three wastewater treatment plants using activated sludge with biological nitrogen removal in different countries: Thailand, United States of America (USA), and Japan. Quantitative real-time polymerase chain reaction (PCR) and PCR coupled with denaturing gradient gel electrophoresis were used to find the comparative abundance and identity of AOB and AOA. The conditions at the Phuket WWTP in Thailand promoted the dominance of AOA amoA genes over AOB amoA genes, while conditions at the WWTPs in Japan and USA promoted growth of AOB. Three parameters that may have contributed to the AOA dominance in Phuket were longer SRT, higher temperature, and higher pH. The Phuket WWTP is a unique system that can be used to better understand the conditions that promote AOA growth and dominance over AOB. In addition, analysis of operational data in conjunction with AOA and AOB community structure from the Phuket WWTP may elucidate advantages of AOA in meeting stricter treatment standards.
在本研究中，利用泰国、美国和日本不同国家具有生物脱氮功能的活性污泥，对三个污水处理厂中氨氧化细菌（AOB）和氨氧化古菌（AOA）中amoA基因的丰度和序列进行了测定。采用定量实时聚合酶链反应（PCR）以及PCR结合变性梯度凝胶电泳来确定AOB和AOA的相对丰度和同一性。泰国普吉岛污水处理厂的条件促使AOA的amoA基因比AOB的amoA基因占优势，而日本和美国污水处理厂的条件则促进了AOB的生长。可能导致普吉岛AOA占优势的三个参数是更长的污泥龄、更高的温度和更高的pH值。普吉岛污水处理厂是一个独特的系统，可用于更好地了解促进AOA生长并使其比AOB占优势的条件。此外，结合普吉岛污水处理厂的运行数据以及AOA和AOB群落结构进行分析，可能会阐明AOA在满足更严格处理标准方面的优势。
