Bae Hyokwan, Chung Yun-Chul, Yang Heejeong, Lee Changsoo, Aryapratama Rio, Yoo Young J, Lee Seockheon
a Center for Water Resource Cycle Research, Korea Institute of Science and Technology (KIST) , Seoul , Republic of Korea.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2015;50(2):201-12. doi: 10.1080/10934529.2014.975550.
In this work, nitrification and changes in the composition of the total bacterial community under inorganic carbon (IC)-limited conditions, in a nitrifying moving bed biofilm reactor, was investigated. A culture-independent analysis of cloning and sequencing based on the 16S rRNA gene was applied to quantify the bacterial diversity and to determine bacterial taxonomic assignment. IC concentrations had significant effects on the stability of ammonia-oxidation as indicated by the reduction of the nitrogen conversion rate with high NH4(+)-N loadings. The predominance of Nitrosomonas europaea was maintained in spite of changes in the IC concentration. In contrast, heterotrophic bacterial species contributed to a high bacterial diversity, and to a dynamic shift in the bacterial community structure, under IC-limited conditions. In this study, individual functions of heterotrophic bacteria were estimated based on taxonomic information. Possible key roles of coexisting heterotrophic bacteria are the assimilation of organic compounds of extracellular polymeric substances produced by nitrifiers, and biofilm formation by providing a filamentous structure and aggregation properties.
在这项研究中，对硝化移动床生物膜反应器中无机碳（IC）受限条件下的硝化作用和总细菌群落组成变化进行了研究。基于16S rRNA基因的非培养克隆和测序分析方法被用于量化细菌多样性并确定细菌的分类归属。如高NH4(+)-N负荷下氮转化率降低所示，IC浓度对氨氧化稳定性有显著影响。尽管IC浓度发生变化，欧洲亚硝化单胞菌仍占主导地位。相比之下，在IC受限条件下，异养细菌种类导致了较高的细菌多样性以及细菌群落结构的动态变化。在本研究中，基于分类信息估计了异养细菌的个体功能。共存异养细菌可能的关键作用是同化硝化菌产生的细胞外聚合物中的有机化合物，以及通过提供丝状结构和聚集特性来形成生物膜。
