Lin Sheng-Yu, Chen Pin-Shiuan, Chang Sarah Y
Department of Chemistry, National Kaohsiung Normal University, Yanchao, Kaohsiung, Taiwan, 824.
J Am Soc Mass Spectrom. 2015 Mar;26(3):530-3. doi: 10.1007/s13361-014-1061-2. Epub 2015 Jan 6.
A simple, rapid, and sensitive method for the detection of posaconazole using dispersive liquid-liquid microextraction (DLLME) coupled to surface-assisted laser desorption/ionization mass spectrometric detection (SALDI/MS) was developed. After the DLLME, posaconazole was detected using SALDI/MS with colloidal gold and α-cyano-4-hydroxycinnamic acid (CHCA) as the co-matrix. Under optimal extraction and detection conditions, the calibration curve, which ranged from 1.0 to 100.0 nM for posaconazole, was observed to be linear. The limit of detection (LOD) at a signal-to-noise ratio of 3 was 0.3 nM for posaconazole. This novel method was successfully applied to the determination of posaconazole in human urine samples.
开发了一种简单、快速且灵敏的方法，用于检测泊沙康唑，该方法采用分散液液微萃取（DLLME）结合表面辅助激光解吸/电离质谱检测（SALDI/MS）。在DLLME之后，使用胶体金和α-氰基-4-羟基肉桂酸（CHCA）作为共基质，通过SALDI/MS检测泊沙康唑。在最佳萃取和检测条件下，观察到泊沙康唑的校准曲线在1.0至100.0 nM范围内呈线性。泊沙康唑在信噪比为3时的检测限（LOD）为0.3 nM。这种新方法成功应用于人体尿液样本中泊沙康唑的测定。
