Department of Chemistry, Lehigh University , Bethlehem, Pennsylvania 18015, United States.
J Am Chem Soc. 2015 Jan 21;137(2):664-6. doi: 10.1021/ja5115437. Epub 2015 Jan 5.
Nearest-neighbor recognition measurements have been made using exchangeable mimics of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine in the liquid-ordered (lo) and liquid-disordered (ld) states. In the ld phase, the net interaction between these two lipids is repulsive. In the lo phase, their interactions are neither attractive nor repulsive. These results, together with previous nearest-neighbor measurements, imply that the overall driving force for lipid domain formation in bilayers composed of high-melting lipids, low-melting lipids, and cholesterol, corresponds to a strong pull (attraction) between the high-melting lipids and cholesterol, a significant push (repulsion) between the low-melting and high-melting lipids, and a significant push between the low-melting lipids and cholesterol. In a broader context, these results provide strong support for the notion that repulsive forces play a major role in the formation of lipid rafts.
已使用 1-棕榈酰基-2-油酰基-sn-甘油-3-磷酸胆碱和 1,2-二棕榈酰基-sn-甘油-3-磷酸胆碱的可交换类似物在有序相 (lo) 和无序相 (ld) 中进行了最近邻识别测量。在 ld 相，这两种脂质之间的净相互作用是排斥的。在 lo 相，它们的相互作用既不吸引人也不排斥。这些结果与以前的最近邻测量结果一起表明，由高熔点脂质、低熔点脂质和胆固醇组成的双层中脂质域形成的总体驱动力对应于高熔点脂质与胆固醇之间的强拉力（吸引力）、低熔点和高熔点脂质之间的显著推力（排斥力），以及低熔点脂质与胆固醇之间的显著推力。从更广泛的角度来看，这些结果为排斥力在脂质筏形成中起主要作用的观点提供了有力支持。
