Schettini G, Landolfi E, Grimaldi M, Meucci O, Postiglione A, Florio T, Ventra C
Institute of Pharmacology, II School of Medicine, University of Naples, Italy.
Biochem Biophys Res Commun. 1989 Nov 30;165(1):496-505. doi: 10.1016/0006-291x(89)91097-8.
The effect of interleukin 1 beta on prolactin secretion and on phosphoinositide turnover in anterior pituitary cells was evaluated. Interleukin 1 beta significantly inhibited TRH-stimulated prolactin secretion assessed by the reverse hemolytic plaque assay. In particular, the cytokine reduced the percentage of plaque forming cells, the plaque mean area, the large plaques percentage. TRH-stimulated inositol phosphate production was also significantly inhibited by interleukin 1 beta. This study shows that interleukin 1 beta reduces TRH-induced prolactin secretion through a direct action on pituitary cell, and attenuates the TRH-stimulated phosphoinositide breakdown. This latter effect may suggest that the reduced lactotropes sensitivity to TRH action may be partially due to interleukin 1 beta inhibition of phosphatidylinositol breakdown.
评估了白细胞介素1β对垂体前叶细胞催乳素分泌及磷酸肌醇代谢的影响。通过反向溶血空斑试验评估，白细胞介素1β显著抑制促甲状腺激素释放激素（TRH）刺激的催乳素分泌。具体而言，该细胞因子降低了空斑形成细胞的百分比、空斑平均面积及大空斑百分比。白细胞介素1β也显著抑制TRH刺激的肌醇磷酸生成。本研究表明，白细胞介素1β通过对垂体细胞的直接作用降低TRH诱导的催乳素分泌，并减弱TRH刺激的磷酸肌醇分解。后一种效应可能提示，促乳素细胞对TRH作用的敏感性降低可能部分归因于白细胞介素1β对磷脂酰肌醇分解的抑制。
