组蛋白甲基转移酶SMYD3在牛卵母细胞成熟和早期胚胎发育过程中调节转录因子的表达。
Histone methyltransferase SMYD3 regulates the expression of transcriptional factors during bovine oocyte maturation and early embryonic development.
作者信息
Bai Haidong, Li Yan, Gao Haixia, Dong Yanhua, Han Pengyong, Yu Haiquan
机构信息
The Key Laboratory of Mammalian Reproductive Biology and Biotechnology, Ministry of Education, Inner Mongolia University, Hohhot, 010021, China.
出版信息
Cytotechnology. 2016 Aug;68(4):849-59. doi: 10.1007/s10616-014-9838-5. Epub 2015 Jan 7.
Mammalian early embryonic development is controlled by a unique program of gene expression, and involves epigenetic reprogramming of histone modifications and DNA methylation. SET and MYND domain-containing protein 3 (SMYD3) is a histone H3 lysine 4 methyltransferase that plays important roles in transcription regulation. The expression of SMYD3 has been studied in some cancer cell lines. However, its expression in oocytes and embryos has not previously been reported. Here, we detected the SMYD3 mRNA and found that it was expressed throughout bovine oocyte in vitro maturation and early embryonic development. Microinjection of SMYD3 siRNA at germinal vesicle stage decreased the transcription level of NANOG, and blocked the development of in vitro fertilization embryos at 4-8 cell stage. Conversely, Microinjection of SMYD3 siRNA at pronuclear stage did not affect early embryonic development. Our findings suggest that SMYD3 regulates the expression of NANOG, and plays an essential role in bovine early embryonic development.
哺乳动物早期胚胎发育受独特的基因表达程序控制，并涉及组蛋白修饰和DNA甲基化的表观遗传重编程。含SET和MYND结构域蛋白3（SMYD3）是一种组蛋白H3赖氨酸4甲基转移酶，在转录调控中起重要作用。已在一些癌细胞系中研究了SMYD3的表达。然而，此前尚未报道其在卵母细胞和胚胎中的表达。在此，我们检测到SMYD3 mRNA，并发现它在牛卵母细胞体外成熟和早期胚胎发育过程中均有表达。在生发泡期显微注射SMYD3 siRNA可降低NANOG的转录水平，并在4-8细胞期阻断体外受精胚胎的发育。相反，在原核期显微注射SMYD3 siRNA不影响早期胚胎发育。我们的研究结果表明，SMYD3调节NANOG的表达，并在牛早期胚胎发育中起重要作用。
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