Lee T Y, Makino K, Shinagawa H, Amemura M, Nakata A
Department of Experimental Chemotherapy, Osaka University, Japan.
J Bacteriol. 1989 Dec;171(12):6593-9. doi: 10.1128/jb.171.12.6593-6599.1989.
The structure and function of the phoB and phoR genes of Shigella dysenteriae strains and Klebsiella pneumoniae, which are involved in regulation of the phosphate regulon, were analyzed. Complementation tests among the genes of Escherichia coli, S. dysenteriae strains, and K. pneumoniae for production of alkaline phosphatase indicate that S. dysenteriae serotype 2 and serotype 3 strains and K. pneumoniae are phoA+ phoB+ phoR+ but S. dysenteriae Sh and serotype 1 strains are phoA phoB+ phoR. Nucleotide sequences of phoB and phoR of S. dysenteriae Sh and K. pneumoniae are highly homologous to those of E. coli, except for a single base insertion found in phoR of S. dysenteriae Sh.
分析了痢疾志贺氏菌菌株和肺炎克雷伯氏菌中参与磷酸盐调节子调控的phoB和phoR基因的结构与功能。大肠杆菌、痢疾志贺氏菌菌株和肺炎克雷伯氏菌的基因间关于碱性磷酸酶产生的互补试验表明，痢疾志贺氏菌2型和3型菌株以及肺炎克雷伯氏菌为phoA+ phoB+ phoR+，但痢疾志贺氏菌Sh和1型菌株为phoA phoB+ phoR。除痢疾志贺氏菌Sh的phoR中发现单个碱基插入外，痢疾志贺氏菌Sh和肺炎克雷伯氏菌的phoB和phoR的核苷酸序列与大肠杆菌的高度同源。
