使用特征序列扩增法（SSAM）扩增和检测微小RNA（miRNA）及非编码RNA（ncRNA）的方法和组合物。
Methods and compositions for amplification and detection of microRNAs (miRNAs) and noncoding RNAs (ncRNAs) using the signature sequence amplification method (SSAM).
作者信息
Ginsberg Stephen D, Che Shaoli
机构信息
Center for Dementia Research, Nathan Kline Institute, NYU Langone Medical Center, 140 Old Orangeburg Road, Orangeburg, NY 10962, 845-398-2170, USA.
出版信息
Recent Adv DNA Gene Seq. 2014;8(1):2-9. doi: 10.2174/2352092208666141001154206.
The signature sequence amplification method (SSAM) described herein is an approach for amplifying noncoding RNA (ncRNA), microRNA (miRNA), and small polynucleotide sequences. A key point of the SSAM technology is the generation of signature sequences. The signature sequences include target sequences (miRNA, ncRNA, and/or any small polynucleotide sequence) flanked by two DNA fragments. Target sequences can be amplified through DNA synthesis, RNA synthesis, or the combination of DNA and RNA synthesis. The amplification of signature sequences provides an efficient and reproducible mechanism to determine the presence or absence of the target miRNAs/ncRNAs, to analyze the quantities of the miRNAs in biological samples, and for miRNA/ncRNA profiling.
本文所述的特征序列扩增方法（SSAM）是一种用于扩增非编码RNA（ncRNA）、微小RNA（miRNA）和小多核苷酸序列的方法。SSAM技术的一个关键点是特征序列的生成。特征序列包括由两个DNA片段侧翼的靶序列（miRNA、ncRNA和/或任何小多核苷酸序列）。靶序列可以通过DNA合成、RNA合成或DNA与RNA合成的组合来扩增。特征序列的扩增提供了一种高效且可重复的机制，用于确定靶miRNA/ncRNA的存在与否、分析生物样品中miRNA的数量以及进行miRNA/ncRNA谱分析。
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