Perry Holly, Henry Stephen
School of Applied Sciences, Faculty of Health and Environmental Sciences, School of Engineering, Faculty of Design and Creative Technologies, Auckland University of Technology, Auckland, New Zealand.
Transfusion. 2015 Jun;55(6 Pt 2):1572-9. doi: 10.1111/trf.12985. Epub 2015 Jan 7.
The ability to recognize and grade serologic reactions in manual techniques remains an important skill both for reference laboratories and in disaster-relocated laboratory services. Developing skills in recognizing and grading serologic reactions is limited to some extent by the range of samples available.
Twenty-six students studying transfusion science were presented with blinded grading panels consisting of mixes of natural cells and kodecytes (natural cells modified with synthetic blood group antigens) representing a range of serologic grades. Results from 15-minute exercises over 17 contact weeks were assessed to determine if training with grading panels would have an impact on the ability of students to recognize and correctly grade serologic reactions. Twenty-one clinically active practitioners also took part in a single analysis.
Grading exercises found that the use of kodecytes and natural negative cells were able to identify deficiencies in both students' and practitioners' ability to recognize negative and grade serologic reactions. The seventeen 15-minute exercises undertaken with students revealed that although there was some improvement in performance in recognizing positive and negative serologic reactions there was also a degradation in ability to accurately grade. Self-assessment showed a major improvement in students' self-efficacy.
The use of serologic grading panels created with kodecytes was suitable as a tool to recognize and monitor serologic grading abilities. Evidence suggests that for both students and practitioners to gain and sustain competency in serologic reaction recognition and grading, they will require ongoing training and monitoring of competence.
对于参考实验室和灾难转移后的实验室服务而言，掌握手工技术中血清学反应的识别和分级能力仍然是一项重要技能。血清学反应识别和分级技能的培养在一定程度上受到可用样本范围的限制。
向26名输血科学专业的学生提供了盲法分级板，这些分级板由天然细胞和编码细胞（用合成血型抗原修饰的天然细胞）的混合物组成，代表一系列血清学分级。对17个接触周内15分钟练习的结果进行评估，以确定使用分级板进行培训是否会对学生识别和正确分级血清学反应的能力产生影响。21名临床执业人员也参与了单次分析。
分级练习发现，使用编码细胞和天然阴性细胞能够识别学生和执业人员在识别阴性和分级血清学反应能力方面的不足。对学生进行的17次15分钟练习表明，虽然在识别阳性和阴性血清学反应方面的表现有一定改善，但准确分级的能力也有所下降。自我评估显示学生的自我效能有了很大提高。
使用由编码细胞创建的血清学分级板作为识别和监测血清学分级能力的工具是合适的。有证据表明，对于学生和执业人员来说，要获得并维持血清学反应识别和分级的能力，他们需要持续的培训和能力监测。
