Gogaladze Khatuna, Chankvetadze Lali, Tsintsadze Maia, Farkas Tivadar, Chankvetadze Bezhan
Institute of Physical and Analytical Chemistry, School of Exact and Natural Sciences, Tbilisi State University, Tbilisi, Georgia.
Chirality. 2015 Mar;27(3):228-34. doi: 10.1002/chir.22417. Epub 2015 Jan 6.
The separation of enantiomers of 16 basic drugs was studied using polysaccharide-based chiral selectors and acetonitrile as mobile phase with emphasis on the role of basic and acidic additives on the separation and elution order of enantiomers. Out of the studied chiral selectors, amylose phenylcarbamate-based ones more often showed a chiral recognition ability compared to cellulose phenylcarbamate derivatives. An interesting effect was observed with formic acid as additive on enantiomer resolution and enantiomer elution order for some basic drugs. Thus, for instance, the enantioseparation of several β-blockers (atenolol, sotalol, toliprolol) improved not only by the addition of a more conventional basic additive to the mobile phase, but also by the addition of an acidic additive. Moreover, an opposite elution order of enantiomers was observed depending on the nature of the additive (basic or acidic) in the mobile phase.
使用基于多糖的手性选择剂，以乙腈作为流动相，研究了16种碱性药物对映体的分离，重点关注碱性和酸性添加剂在对映体分离和洗脱顺序中的作用。在所研究的手性选择剂中，与纤维素苯基氨基甲酸酯衍生物相比，基于直链淀粉苯基氨基甲酸酯的手性选择剂更常表现出手性识别能力。对于某些碱性药物，观察到甲酸作为添加剂对映体拆分和对映体洗脱顺序有有趣的影响。因此，例如，几种β受体阻滞剂（阿替洛尔、索他洛尔、托利洛尔）的对映体分离不仅通过向流动相中添加更传统的碱性添加剂得到改善，而且通过添加酸性添加剂也得到改善。此外，根据流动相中添加剂（碱性或酸性）的性质，观察到对映体的洗脱顺序相反。
