Vidal-Quist J C, Ortego F, Lombardero M, Castañera P, Hernández-Crespo P
Laboratorio de Interacción Planta-Insecto, Departamento de Biología Medioambiental, Centro de Investigaciones Biológicas - CSIC, Madrid, Spain.
Med Vet Entomol. 2015 Jun;29(2):137-46. doi: 10.1111/mve.12102. Epub 2015 Jan 7.
House dust mites are a major source of allergy worldwide. While diagnosis and treatment based on mite extracts have remarkably advanced, little information exists on the expression of allergens in mites. We have studied gene expression of eight Dermatophagoides pteronyssinus (Trouessart) (Acari: Pyroglyphidae) allergens (Der p 1, 2, 3, 4, 5, 7, 10 and 21). All allergens showed higher transcription in nymphs compared with larvae or adults, with the only exception of Der p 10. The transcription of Der p 4 and Der p 10, together with the transcription and protein ratios Der p 1 to Der p 2, were higher in males than in females. One-week exposure of mite cultures to 16 or 35 °C (versus 24 °C) or low RH (44% versus 76%) significantly influenced the allergen gene transcription profile. Our results demonstrate that allergen expression is quantitatively and/or qualitatively influenced by mite development and sex, as well as by the environment. We suggest that monitoring allergen gene expression may be a useful tool to assist the optimization of mite cultures in the production of standardized allergenic extracts for clinical use.
屋尘螨是全球过敏的主要来源。虽然基于螨提取物的诊断和治疗已经取得了显著进展，但关于螨中变应原的表达情况却知之甚少。我们研究了八种粉尘螨（Trouessart）（蜱螨亚纲：粉螨科）变应原（Der p 1、2、3、4、5、7、10和21）的基因表达。与幼虫或成虫相比，所有变应原在若虫中的转录水平更高，Der p 10除外。Der p 4和Der p 10的转录，以及Der p 1与Der p 2的转录和蛋白质比率，在雄性中高于雌性。将螨培养物在16或35°C（相对于24°C）或低相对湿度（44%相对于76%）下暴露一周，显著影响了变应原基因转录谱。我们的结果表明，变应原表达在数量和/或质量上受到螨的发育、性别以及环境的影响。我们建议，监测变应原基因表达可能是一种有用的工具，有助于优化螨培养物，以生产用于临床的标准化变应原提取物。
