Awad M, Gavish M
Rappaport Family Institute for Research in the Medical Sciences, Faculty of Medicine, Technion-Israel Institute of Technology, Haifa.
J Recept Res. 1989;9(4-5):369-84. doi: 10.3109/10799898909066064.
The effect of various detergents treatment on the specific binding of [3H]PK 11195 (2nM) to peripheral-type benzodiazepine binding sites (PBS) in calf and rat kidney, adrenal gland, and cerebral cortex membranes was studied. At a concentration of 0.025%, Triton X-100 increased [3H]PK 11195 specific binding to calf kidney, adrenal gland, and cerebral cortex membranes by 20-40%. At the same concentration, Triton X-100 scarcely affected specific binding of [3H]PK 11195 to rat cerebral cortex but decreased binding to rat kidney and adrenal gland membranes by 20-30%. At a concentration of 0.05% of Triton X-100, [3H]PK 11195 specific binding to calf kidney, adrenal gland, and cerebral cortex membranes was increased by 10-20%; whereas [3H]PK 11195 specific binding to rat kidney, adrenal gland, and cerebral cortex membranes was decreased by more than 40%. The increase in [3H]PK 11195 specific binding to calf kidney membranes following Triton X-100 (0.05%) treatment was apparently due to an increase in the binding affinity of PBS, since the density remained unaltered; whereas, the decrease in [3H]PK 11195 specific binding to rat kidney membranes was due to a decrease in both binding affinity and density of PBS. On the other hand, the detergents 3- [(3- cholamidopropyl)- dimethylammonio] - 1 - propane sulfonate (CHAPS), Tween 20, deoxycholic acid, and digitonin have a similar effect on [3H]PK 11195 specific binding to PBS in both calf and rat kidney membranes.
研究了各种去污剂处理对[3H]PK 11195（2nM）与小牛和大鼠肾脏、肾上腺及大脑皮层膜中外周型苯二氮䓬结合位点（PBS）特异性结合的影响。在0.025%的浓度下，Triton X-100使[3H]PK 11195与小牛肾脏、肾上腺及大脑皮层膜的特异性结合增加了20%-40%。在相同浓度下，Triton X-100几乎不影响[3H]PK 11195与大鼠大脑皮层的特异性结合，但使与大鼠肾脏和肾上腺膜的结合减少了20%-30%。在0.05%的Triton X-100浓度下，[3H]PK 11195与小牛肾脏、肾上腺及大脑皮层膜的特异性结合增加了10%-20%；而[3H]PK 11195与大鼠肾脏、肾上腺及大脑皮层膜的特异性结合减少了40%以上。Triton X-100（0.05%）处理后[3H]PK 11195与小牛肾脏膜特异性结合的增加显然是由于PBS结合亲和力的增加，因为密度保持不变；而[3H]PK 11195与大鼠肾脏膜特异性结合的减少是由于PBS结合亲和力和密度的降低。另一方面，去污剂3-[(3-胆酰胺丙基)-二甲基铵]-1-丙烷磺酸盐（CHAPS）、吐温20、脱氧胆酸和洋地黄皂苷对[3H]PK 11195与小牛和大鼠肾脏膜中PBS的特异性结合有类似影响。
