[乙肝慢性患者脾胃湿热证与肝郁脾虚证中微小RNA的差异表达：一项初步研究]

[Differential expression of microRNA in chronic hepatitis B patients of pi-wei dampness-heat syndrome and of gan depression Pi deficiency syndrome: a primary research].

作者信息

Wang En-Cheng, Zhang Lei, Liu Hong-Wei, Guo Yin-Ling, Zhang Feng, He Cheng-Wei, Shen Meng-Meng, Feng Quan-Sheng

机构信息

Department of Gastroenterology, Pixian Chinese Medicine, Hospital, Sichuan, China.

出版信息

Zhongguo Zhong Xi Yi Jie He Za Zhi. 2014 Nov;34(11):1324-8.

PMID:25566623

Abstract

OBJECTIVE

To explore different microRNA expression profiles between chronic hepatitis B (CHB) patients of Pi-Wei dampness-heat syndrome (PWDHS) and Gan depression Pi deficiency syndrome (GDPDS).

METHODS

By applying gene chip technology, blood samples from CHB patients of PWDHS (3 cases), GDPDS (3 cases), and healthy volunteers (3 cases) were withdrawn and microRNA detected. The microRNA was screened and functional analyses performed by using SAS system.

RESULTS

Totally 77 microRNAs with differential expression were screened from CHB patients of PWDHS and healthy volunteers, including 60 up-regulated microRNAs and 17 down-regulated microRNAs. Functions of target genes were mainly associated with transcription factors, gas exchange, adverse stimulating, regulation of enzyme activities, developing of the immune system, and the process of actin filaments. Totally 41 microRNAs with differential expression were screened from CHB patients of GDPDS and healthy volunteers, including 32 up-regulated microRNAs and 9 down-regulated microRNAs. Functions of target genes were mainly associated with binding to nucleotide or chromatin, inhibition and activation of transcription, biosynthesis, regulation of metabolic process, regulation of enzyme activities, developing of the immune system, the process of actin filaments, and IL-12. Totally 6 microRNAs with differential expression were screened from CHB patients of PWDHS and CHB patients of GDPDS, including 1 up-regulated microRNA and 5 down-regulated microRNAs. Functions of target genes were mainly associated with transmembrane transport, regulation of transcription factors, metabolism of hormones, developing of the immune system, the process of actin filaments, regulation of metabolic process, response to exterior stimulation, and so on.

CONCLUSION

There existed differentially expressed microRNAs (spectrum) between CHB patients of PWDHS and CHB patients of GDPDS.

摘要

目的

探讨慢性乙型肝炎（CHB）脾虚湿热证（PWDHS）患者与肝郁脾虚证（GDPDS）患者之间不同的微小RNA表达谱。

方法

应用基因芯片技术，采集PWDHS的CHB患者（3例）、GDPDS的CHB患者（3例）及健康志愿者（3例）的血液样本并检测微小RNA。使用SAS系统对微小RNA进行筛选及功能分析。

结果

从PWDHS的CHB患者与健康志愿者中筛选出77个差异表达的微小RNA，其中上调的微小RNA有60个，下调的微小RNA有17个。靶基因功能主要与转录因子、气体交换、不良刺激、酶活性调节、免疫系统发育及肌动蛋白丝形成过程等有关。从GDPDS的CHB患者与健康志愿者中筛选出41个差异表达的微小RNA，其中上调的微小RNA有32个，下调的微小RNA有9个。靶基因功能主要与核苷酸或染色质结合、转录抑制与激活、生物合成、代谢过程调节、酶活性调节、免疫系统发育、肌动蛋白丝形成过程及白细胞介素-12等有关。从PWDHS的CHB患者与GDPDS的CHB患者中筛选出6个差异表达的微小RNA，其中上调的微小RNA有1个，下调的微小RNA有5个。靶基因功能主要与跨膜转运、转录因子调节、激素代谢、免疫系统发育、肌动蛋白丝形成过程、代谢过程调节、对外界刺激的反应等有关。