Dong Ke-Zhou, Zhu Yi-Min
Department of Respiratory Medicine, Second Hospital of Traditional Chinese Medicine, Nanjing, China.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2014 Nov;34(11):1342-6.
To observe the expression of tumor necrosis factor-alpha (TNF-α) protein in the lung tissue of rats with chronic obstructive pulmonary disease (COPD), and to evaluate the intervention and mechanism of Heche Chongcao Capsule (HCC).
The COPD rat model was prepared by exposure to cigarettes smoke plus intratracheal injecting lipopolysaccharide (LPS). Forty successfully modeled SD rats were randomly divided into the COPD model group, the control group, the low dose HCC group, the medium dose HCC group, and the high dose HCC group, 8 in each group. Meanwhile, a normal control group consisting of 6 rats was also set up. HCC at 0.25, 0.5, and 1.0 g/kg was administered to rats in the 3 dose HCC groups respectively by gastrogavage combined with Theophylline Sustained Release Tablet (TSRT). Rats in the control group were administered with TSRT by gastrogavage at 4.5 mg/kg, 1 mL/100 g each time, once daily. All medication lasted for 4 successive weeks. Equal volume of distilled water was administered by gastrogavage to rats in the COPD model group and the normal control group. Morphological changes of the lung tissue were observed under microscope. The expression of TNF-α protein in the lung tissue were also detected using Real-time PCR.
Under electron microscope, the cilium in the tracheal epithelium was disorderly arranged, type I and II alveolar cells were degenerated, endoplasmic reticulum and mitochondria were swollen, the lamellar body was emptied, and free fragment could be seen inside alveolar space. Compared with the model group, all lesions were somewhat ameliorated in all medicated groups, especially in the medium dose HCC group. Compared with the model group, the expression of TNF-α protein decreased in all medicated groups, especially in the medium and low dose HCC groups (P < 0.05, P < 0.01). Compared with the control group, the expression of TNF-α protein decreased in the medium and low dose HCC groups (P < 0.05).
HCC could effectively regulate the expression of TNF-α protein and inhibit airway inflammation reaction in COPD rats.
观察慢性阻塞性肺疾病（COPD）大鼠肺组织中肿瘤坏死因子-α（TNF-α）蛋白的表达，评价诃车虫草胶囊（HCC）的干预作用及机制。
采用香烟烟雾暴露加气管内注射脂多糖（LPS）制备COPD大鼠模型。将40只造模成功的SD大鼠随机分为COPD模型组、对照组、HCC低剂量组、HCC中剂量组、HCC高剂量组，每组8只。同时设立由6只大鼠组成的正常对照组。3个HCC剂量组大鼠分别以0.25、0.5、1.0 g/kg的HCC灌胃，同时给予茶碱缓释片（TSRT）。对照组大鼠以4.5 mg/kg的TSRT灌胃，每次1 mL/100 g，每日1次。所有药物连续给药4周。COPD模型组和正常对照组大鼠灌胃等量蒸馏水。显微镜下观察肺组织形态学变化。采用实时荧光定量PCR检测肺组织中TNF-α蛋白的表达。
电镜下可见气管上皮纤毛排列紊乱，Ⅰ型和Ⅱ型肺泡细胞变性，内质网和线粒体肿胀，板层小体排空，肺泡腔内可见游离碎片。与模型组相比，各用药组的所有病变均有不同程度改善，尤以HCC中剂量组为著。与模型组相比，各用药组TNF-α蛋白表达均降低，尤以HCC中、低剂量组明显（P<0.05，P<0.01）。与对照组相比，HCC中、低剂量组TNF-α蛋白表达降低（P<0.05）。
HCC可有效调节COPD大鼠TNF-α蛋白的表达，抑制气道炎症反应。
