Isolation and purification of two antioxidant peptides from alcalase hydrolysate of Arca subcrenata.

OBJECTIVE

To investigate the constituents with antioxidant activities from alcalase hydrolysate of Arca subcrenata.

METHODS

The consecutive chromatographic methods were employed,including ion-exchange chromatography, gel filtration chromatography, and reverse phase high-performance liquid chromatography (RP-HPLC). The amino acid sequences of the purified antioxidant peptides were determined by automated Edman degradation.

RESULTS

Under the guidance of the assay of scavenging free radicals, two peptides with antioxidant activities, termed as A-Bg1 and A-Bh, were isolated and purified from the alcalase hydrolysate of Arca subcrenata.

CONCLUSION

Constituents from the hydrolysate of Arca subcrenata might be a new potential resource of antioxidants.