Huang Qiong, Xu Hai Bin, Yu Zhou, Liu Shan, Gao Peng
Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 511430, Guangdong China; Key Laboratory of Food Safety Risk Assessment of Ministry of Health, China National Center for Food Safety Risk Assessment, Beijing 100022, China.
Key Laboratory of Food Safety Risk Assessment of Ministry of Health, China National Center for Food Safety Risk Assessment, Beijing 100022, China.
Biomed Environ Sci. 2015 Jan;28(1):85-8. doi: 10.3967/bes2015.010.
By now, the digestive stability experiments provided by most authoritative organizations are in vitro tests. Evaluating the protein digestive stability with in vivo models should be more objective. The present study aimed to verify the in vivo digestibility of soybean β-conglycinin β-subunit in Wuzhishan (WZS) minipigs. Three minipigs were surgically fitted with O-stomach and T-ileum cannulae and fed with soybean meals. According to SDS-PAGE, the 50 kD fraction of soybean β-conglycinin β-subunit persisted in the gastric fluid until 6 h after feeding, which was detected at 3 h and clearly visible at 4-6 h in the intestinal fluid. Western blot with anti-β-conglycinin β-subunit McAb confirmed it.
目前，大多数权威机构提供的消化稳定性实验都是体外试验。用体内模型评估蛋白质的消化稳定性应该更客观。本研究旨在验证五指山（WZS）小型猪体内大豆β-伴大豆球蛋白β亚基的消化率。对三只小型猪进行手术，安装O型胃和T型回肠插管，并喂食豆粕。根据SDS-PAGE分析，大豆β-伴大豆球蛋白β亚基的50 kD组分在胃液中持续存在至喂食后6小时，在肠液中3小时可检测到，4-6小时清晰可见。用抗β-伴大豆球蛋白β亚基单克隆抗体进行的蛋白质印迹分析证实了这一点。
