番鸭产蛋性能与生长激素（GH）、催乳素（PRL）和垂体特异性转录因子1（Pit-1）基因5'-侧翼区多态性的关系

Polymorphisms in the 5'-flanking regions of the GH, PRL, and Pit-1 genes with Muscovy duck egg production.

作者信息

Xu Z Q, He J, Ji C L, Zhang Y, Nie Q H, Zhang D X, Zhang X Q

机构信息

Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, and Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, South China Agricultural Univ., Guangdong 510642, P. R. China.

出版信息

J Anim Sci. 2015 Jan;93(1):28-34. doi: 10.2527/jas.2014-8071.

DOI:10.2527/jas.2014-8071

PMID:25568353

Abstract

Somatotropic axis-related genes contribute to reproduction of ducks. Five SNP in the 5'-flanking regions of the growth hormone (GH), prolactin (PRL), and pituitary-specific transcription factor (Pit-1) genes were identified and genotyped in a female population of Muscovy ducks. Association analysis of these SNP with Muscovy duck egg production traits was performed. Results showed that SNP C-515G of GH was significantly associated with egg number in ducks at age 59 wk (E59W; P = 0.0009) and egg number in ducks at age 300 d (E300D; P = 0.0022). Single nucleotide polymorphism C-441T of GH was significantly associated with E59W (P = 0.0014). Significant associations of SNP T-884C and T-335C of PRL with the age at first egg (A1D), E59W, and E300D were detected in this population (P < 0.0001). It was concluded that these 4 SNP might be useful markers to use with the aim of increasing Muscovy duck E59W. On the basis of genetic parameter estimation, the heritability of A1D, E300D, E59W, and molting time were 0.43 ± 0.04, 0.45 ± 0.04, 0.36 ± 0.04, and 0.04 ± 0.03, respectively. Strong positive genetic correlation was noted between E59W and E300D (correlation coefficient = 0.80), whereas a negative association was noted between E59W and A1D (correlation coefficient = -0.80). Therefore, the selection for improved A1D should also increase E59W.

摘要

生长激素轴相关基因对鸭的繁殖有影响。在番鸭母鸭群体中，对生长激素（GH）、催乳素（PRL）和垂体特异性转录因子（Pit-1）基因5'侧翼区域的5个单核苷酸多态性（SNP）进行了鉴定和基因分型。对这些SNP与番鸭产蛋性状进行了关联分析。结果表明，GH基因的SNP C-515G与59周龄鸭的产蛋数（E59W；P = 0.0009）和300日龄鸭的产蛋数（E300D；P = 0.0022）显著相关。GH基因的单核苷酸多态性C-441T与E59W显著相关（P = 0.0014）。在该群体中检测到PRL基因的SNP T-884C和T-335C与开产日龄（A1D）、E59W和E300D显著相关（P < 0.0001）。得出结论，这4个SNP可能是用于提高番鸭E59W的有用标记。基于遗传参数估计，A1D、E300D、E59W和换羽时间的遗传力分别为0.43±0.04、0.45±0.04、0.36±0.04和0.04±0.03。E59W和E300D之间存在强正遗传相关性（相关系数 = 0.80），而E59W和A1D之间存在负相关性（相关系数 = -0.80）。因此，选择改善A1D也应能提高E59W。