Raeisi Mojtaba, Tajik Hossein, Aliakbarlu Javad, Valipour Sima
Department of Public Health, Faculty of Health, Golestan University of Medical Sciences, Gorgan, Iran;
Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran;
Vet Res Forum. 2014 Spring;5(2):89-93.
Meat products, especially fish meat, are very susceptible to lipid oxidation and microbial spoilage. In this study, first, gas chromatography mass spectrometry (GC-MS) analysis of Zataria multiflora essential oil (ZEO) components was done and then two concentrations of ZEO, (1% and 2%) and two concentrations of grape seed extract (GSE), (0.5% and 1%) were used in carboxymethyl cellulose coating alone and in combination, and their antioxidant effects on rainbow trout meat were evaluated in a 20-day period using thiobarbituric acid reactive substances (TBARS) test. Their effects on total volatile basic nitrogen (TVBN) and pH were evaluated as well. The main components of ZEO are thymol and carvacrol. These components significantly decreased production of thiobarbituric acid (TBA), TVBN and pH level of fish meat. The initial pH, TVBN and TBA content was 6.62, 12.67 mg N per 100 g and 0.19 mg kg(-1), respectively. In most treatments significant (p < 0.05) effects on aforementioned factors was seen during storage at 4 ˚C. The results indicated that use of ZEO and GSE as a natural antioxidant agents was effective in reducing undesirable chemical reactions in storage of fish meat.
肉类产品,尤其是鱼肉,极易发生脂质氧化和微生物腐败。在本研究中,首先对百里香精油(ZEO)的成分进行了气相色谱 - 质谱联用(GC - MS)分析,然后单独以及组合使用两种浓度的ZEO(1%和2%)和两种浓度的葡萄籽提取物(GSE,0.5%和1%)添加到羧甲基纤维素涂层中,并在20天的时间内使用硫代巴比妥酸反应物(TBARS)试验评估它们对虹鳟鱼肉的抗氧化作用。同时也评估了它们对总挥发性盐基氮(TVBN)和pH值的影响。ZEO的主要成分是百里香酚和香芹酚。这些成分显著降低了鱼肉中硫代巴比妥酸(TBA)、TVBN的生成以及pH值。鱼肉的初始pH值、TVBN和TBA含量分别为6.62、每100克12.67毫克氮和0.19毫克/千克。在大多数处理中,在4℃储存期间,对上述因素有显著(p < 0.05)影响。结果表明,使用ZEO和GSE作为天然抗氧化剂可有效减少鱼肉储存过程中不良的化学反应。