一项关于发情周期对奶牛子宫液和血清免疫球蛋白G（IgG）含量影响的研究。
A study on the effects of the estrous cycle on uterine fluid and blood serum immunoglobulin G (IgG) content in the cow.
作者信息
Alavi-Shoushtari Sayed Mortaza, Abedizadeh Roya, Khaki Amir, Mokarizadeh Aram, Dorostkar Kamran
机构信息
Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran;
Department of Immunology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran;
出版信息
Vet Res Forum. 2014 Spring;5(2):115-9.
To investigate the IgG content and its variations in uterine fluid (UF) during the estrous cycle of the cow and to compare them with those of the blood serum (S), six pairs of serum and UF samples for each phase of the cycle selected out of 240 bovine genital tracts and blood samples were collected from Urmia abattoir. The UF samples were collected by gentle scraping of the endometrium using a curette after uterine incision and their IgG content and those of the serum were measured by single radial immuno-diffusion (SRID) assay. Serum IgG values (Mean ± SEM) were generally higher than the UF values throughout the cycle except for di-estrus (S: 38.50 ± 0.90, UF: 51.60 ± 2.10 mg mL(-1)), in which the highest values were observed in UF samples. In met-estrus the difference was not significant (S: 34.80 ± 1.80 mg mL(-1), UF: 30.80 ± 5.20 mg mL(-1)), however, in estrus the mean UF IgG value (12.50 ± 1.10 mg mL(-1)) was lower than that of the serum (31.30 ± 1.20 mg mL(-1)). In pro-estrus, the lowest values (S: 27.80 ± 1.30 mg mL(-1), UF: 9.10 ± 1.50 mg mL(-1)) were obtained. The results showed a lower IgG values in the bovine UF than those of the serum in the follicular phase of the cycle, while in di-estrus the UF IgG content was the highest, suggesting some IgG production in the uterus at this phase.
为了研究奶牛发情周期中子宫液（UF）的IgG含量及其变化，并将其与血清（S）的IgG含量进行比较，从乌尔米耶屠宰场收集了240份牛生殖道和血液样本，为每个发情周期阶段选取6对血清和子宫液样本。子宫液样本是在子宫切开后用刮匙轻轻刮取子宫内膜收集的，其IgG含量和血清的IgG含量通过单向放射免疫扩散（SRID）测定法进行测量。除了间情期（S：38.50±0.90，UF：51.60±2.10mg mL⁻¹）外，整个发情周期中血清IgG值（平均值±标准误）通常高于子宫液值，在间情期子宫液样本中观察到最高值。在发情后期差异不显著（S：34.80±1.80mg mL⁻¹，UF：30.80±5.20mg mL⁻¹），然而，在发情期子宫液平均IgG值（12.50±1.10mg mL⁻¹）低于血清（31.30±1.20mg mL⁻¹）。在发情前期，获得了最低值（S：27.80±1.30mg mL⁻¹，UF：9.10±1.50mg mL⁻¹）。结果表明，在发情周期的卵泡期，牛子宫液中的IgG值低于血清中的IgG值，而在间情期子宫液IgG含量最高，表明在此阶段子宫有一些IgG产生。
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