Bouzari Majid
Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran.
Vet Res Forum. 2014 Spring;5(2):145-8.
Experimental infection of Muscovy ducks with V4 strain of Newcastle disease virus was undertaken to determine the response of the ducks to the virus and the possibility of virus transmission to ducks and chickens in village like conditions. Twelve ducks were randomly and equally divided into three groups of control, inoculated and in-contact. Additionally, the chickens were placed into two groups of four animals each, namely in-contact and control. The inoculated and in-contact ducks and in-contact chickens were kept together. The eye drop route was used for inoculation and hemagglutination inhibition (HI) antibodies were measured for assessment of antibody response and cloacal and pharyngeal swabs were used for detection of the virus. The primary antibody response of inoculated ducks was very high and rapid (geometric mean titers [Log base 2] of up to 5.75 ± 0.50). The in-contact ducks showed antibody response with the same pattern but lower titers than the inoculated ducks (geometric mean titers [Log base 2] of up to 3.25 ± 1.70). The in-contact chickens showed a slight increase of HI antibody (geometric mean titers [Log base 2] of up to 2.25 ± 1.25) while the control chickens did not show any increase. The antibody response indicated the transmission of the virus to contact ducks and chickens. A single isolation of virus confirmed the ability of ducks to excrete the virus. It was concluded that the V4 strain of Newcastle disease virus was highly antigenic for ducks, and ducks can transmit it to other ducks and also in-contact chickens.
用新城疫病毒V4株对番鸭进行实验性感染，以确定番鸭对该病毒的反应以及在类似乡村环境中该病毒传播给番鸭和鸡的可能性。12只番鸭被随机且等分为三组：对照组、接种组和接触组。此外，将鸡分为两组，每组4只，即接触组和对照组。接种组和接触组的番鸭以及接触组的鸡饲养在一起。采用滴眼途径进行接种，并检测血凝抑制（HI）抗体以评估抗体反应，同时采集泄殖腔和咽拭子检测病毒。接种组番鸭的初次抗体反应非常高且迅速（几何平均滴度[以2为底的对数]高达5.75±0.50）。接触组番鸭呈现相同模式的抗体反应，但滴度低于接种组番鸭（几何平均滴度[以2为底的对数]高达3.25±1.70）。接触组鸡的HI抗体略有增加（几何平均滴度[以2为底的对数]高达2.25±1.25），而对照组鸡未出现任何增加。抗体反应表明病毒传播给了接触组的番鸭和鸡。单次病毒分离证实了番鸭具有排出病毒的能力。得出的结论是，新城疫病毒V4株对番鸭具有高度抗原性，番鸭可将其传播给其他番鸭以及接触组的鸡。
