Chamieh Joseph, Martin Michel, Cottet Hervé
Institut des Biomolécules Max Mousseron (IBMM, UMR 5247 CNRS-Université de Montpellier 1, Université de Montpellier 2) , Place Eugène Bataillon CC 1706, 34095 Montpellier Cedex 5, France.
Anal Chem. 2015 Jan 20;87(2):1050-7. doi: 10.1021/ac503789s. Epub 2015 Jan 8.
Quantitative analysis in capillary electrophoresis based on time-scale electropherograms generally uses time-corrected peak areas to account for the differences in apparent velocities between solutes. However, it could be convenient and much more relevant to change the time-scale electropherograms into mass relative distribution of the effective mobility or any other characteristic parameter (molar mass, chemical composition, charge density, ...). In this study, the theoretical background required to perform the variable change on the electropherogram was developed with an emphasis on the fact that both x and y axes should be changed when the time scale electropherograms are modified to get the distributions. Applications to the characterization of polymers and copolymers by different modes of capillary electrophoresis (CE) are presented, including the molar mass distribution of poly-L-lysine oligomers by capillary gel electrophoresis (CGE), molar mass distribution of end-charged poly-l-alanine by free solution CE, molar mass distribution of evenly charged polyelectrolytes by CGE, and charge density distribution of variously charged polyelectrolytes by free solution CE.
基于时间尺度电泳图的毛细管电泳定量分析通常使用时间校正峰面积来解释溶质之间表观速度的差异。然而，将时间尺度电泳图转换为有效迁移率或任何其他特征参数（摩尔质量、化学成分、电荷密度等）的质量相对分布可能会更方便且更具相关性。在本研究中，开发了在电泳图上进行变量转换所需的理论背景，重点强调了在修改时间尺度电泳图以获得分布时，x轴和y轴都应进行转换。本文展示了通过不同模式的毛细管电泳（CE）对聚合物和共聚物进行表征的应用，包括通过毛细管凝胶电泳（CGE）对聚-L-赖氨酸低聚物的摩尔质量分布分析、通过自由溶液CE对末端带电聚-L-丙氨酸的摩尔质量分布分析、通过CGE对均匀带电聚电解质的摩尔质量分布分析以及通过自由溶液CE对不同电荷聚电解质的电荷密度分布分析。
