de Oliveira Katharina Morant Holanda, da Silva Raquel Assed Bezerra, Küchler Erika Calvano, de Queiroz Alexandra Mussolino, Nelson Filho Paulo, da Silva Lea Assed Bezerra
Department of Pediatric Clinic, School of Dentistry of Ribeirão Preto, University of São Paulo , Ribeirão Preto , Brazil.
Ultrastruct Pathol. 2015 May;39(3):187-91. doi: 10.3109/01913123.2014.975389. Epub 2015 Jan 8.
Periapical lesions are processes mediated from bacterial invasion on the pulp tissue, which results in inflammatory process and bone resorption. Animal models have been used for the study of cell interactions and inflammatory responses involved in periapical lesion. The use of different methods for morphometric evaluation of this lesion has been reported. However, due to the limitation of 2D methods, computerized microtomography (µCT) has been indicated. Therefore, the aim of this study was to evaluate the correlation between the histomorphometric measures of periapical lesions and the µCT analysis obtained in an animal experimental model. Briefly, the periapical lesions were induced by pulp exposal and after the experimental periods (7, 21 and 42 days) the mice were euthanized. The mandibles were evaluated under the µCT scanning and then were subjected to the routine histologic procedures, stained with hematoxilin and eosin. The periapical lesions were measured by the specific softwares of each technique. A weak correlation among the histomorphometric measurement and the 2D µCT analysis (r(2)= .204) and between the histomorphometric measurement and the 3D µCT analysis (r(2)= .237) was observed. A strong correlation between the 2D and 3D µCT analysis (r(2)= .867) was observed. Thereby, it can be concluded that the µCT is a method that allows a precise volumetric scanning and represents an important tool for morphometric evaluation.
根尖周病变是由细菌侵入牙髓组织介导的过程，这会导致炎症反应和骨吸收。动物模型已被用于研究根尖周病变中涉及的细胞相互作用和炎症反应。已有报道使用不同方法对该病变进行形态计量学评估。然而，由于二维方法的局限性，计算机微断层扫描（µCT）已被推荐使用。因此，本研究的目的是评估在动物实验模型中获得的根尖周病变组织形态计量学测量值与µCT分析之间的相关性。简要地说，通过暴露牙髓诱导根尖周病变，在实验期（7天、21天和42天）后对小鼠实施安乐死。对下颌骨进行µCT扫描评估，然后进行常规组织学程序，用苏木精和伊红染色。通过每种技术的特定软件测量根尖周病变。观察到组织形态计量学测量值与二维µCT分析之间的弱相关性（r(2)=0.204）以及组织形态计量学测量值与三维µCT分析之间的弱相关性（r(2)=0.237）。观察到二维和三维µCT分析之间的强相关性（r(2)=0.867）。因此，可以得出结论，µCT是一种能够进行精确容积扫描的方法，是形态计量学评估的重要工具。
