Muallem S, Merritt B S, Green J, Kleeman C R, Yamaguchi D T
Department of Physiology, University of Texas Southwestern Medical Center, Dallas 75235.
Biochem J. 1989 Nov 1;263(3):769-74. doi: 10.1042/bj2630769.
A wide spectrum of prostaglandins (PG) stimulate both the production of cyclic AMP and an increase in free cytosolic Ca2+ concentration [( Ca2+]i) in the osteogenic osteosarcoma cell line, UMR-106-01, which has characteristics compatible with osteoblasts. Using PG-stimulated determinations of the second messengers cyclic AMP and [Ca2+]i, a method for classification of PG receptors is presented. UMR-106-01 cells demonstrate three subclasses of PG receptors. One receptor interacts with PGF2 alpha, PGD2, and thromboxane B2 (TxB2) to increase [Ca2+]i. A second receptor binds PGE2, PGE1, PGI2, PGA2 and 6-oxo-PGF1 alpha to increase [Ca2+]i by stimulation of a second separate phospholipase C pool. A third receptor accepts PGE2, PGE1, PGA2, PGI2 and to a lesser extent PGF2 alpha, PGD2 and TxB2 to increase cyclic AMP. Such a classification system may be applicable to other cells responding to multiple PGs by inducing changes in cellular second messengers.
多种前列腺素（PG）可刺激具有成骨细胞特性的成骨性骨肉瘤细胞系UMR-106-01中环状AMP的产生以及游离胞质钙离子浓度[Ca2+]i的升高。利用PG刺激下对第二信使环状AMP和[Ca2+]i的测定，提出了一种PG受体分类方法。UMR-106-01细胞显示出PG受体的三个亚类。一种受体与前列腺素F2α、前列腺素D2和血栓素B2（TxB2）相互作用，以增加[Ca2+]i。第二种受体结合前列腺素E2、前列腺素E1、前列环素I2、前列腺素A2和6-氧代前列腺素F1α，通过刺激第二个独立的磷脂酶C池来增加[Ca2+]i。第三种受体接受前列腺素E2、前列腺素E1、前列腺素A2、前列环素I2，在较小程度上还接受前列腺素F2α、前列腺素D2和血栓素B2，以增加环状AMP。这样的分类系统可能适用于其他通过诱导细胞第二信使变化来响应多种PG的细胞。
