Abacı Ayhan, Çatlı Gönül, Anık Ahmet, Küme Tuncay, Çalan Özlem Gürsoy, Dündar Bumin Nuri, Böber Ece
Department of Pediatric Endocrinology, Dokuz Eylul University, Faculty of Medicine, Izmir, Turkey.
Department of Pediatric Endocrinology, Dokuz Eylul University, Faculty of Medicine, Izmir, Turkey.
Peptides. 2015 Feb;64:29-33. doi: 10.1016/j.peptides.2014.12.011. Epub 2015 Jan 6.
The aim of the present study was to investigate the diagnostic role of serum neurokinin B level and its relationship with kisspeptin and leptin, which are known to be involved in the initiation of pubertal process. Girls who presented with breast development (<8 years) were included in the study. All patients underwent bone age assessment. Basal levels of serum follicle stimulating hormone and luteinizing hormone were measured and gonadotropin releasing hormone stimulation test was performed. Patients with a bone age/chronological age ratio >1 and a peak luteinizing hormone response in gonadotropin releasing hormone stimulation test >5mIU/L were included in the central precocious puberty group, while patients who did not meet these criteria were included in the premature thelarche group. Patients with organic pathologies were excluded. Healthy prepubertal girls with similar age were included as the control group. Leptin, kisspeptin and neurokinin B levels were measured by ELISA method. The study included 20 girls with idiopathic central precocious puberty 22 girls with premature thelarche and 24 prepubertal controls. While serum kisspeptin, leptin and neurokinin B levels were significantly higher in central precocious puberty and premature thelarche groups compared to controls, no significant difference was found between central precocious puberty and premature thelarche groups. Increased serum levels of leptin, kisspeptin and neurokinin B in patients with premature thelarche and central precocious puberty suggest that they take part during the initiation of pubertal process, however, these markers are not able to differentiate patients with central precocious puberty from premature thelarche.
本研究的目的是探讨血清神经激肽B水平的诊断作用及其与已知参与青春期启动过程的亲吻素和瘦素的关系。纳入乳房发育（<8岁）的女孩进行研究。所有患者均接受骨龄评估。测量血清促卵泡生成素和促黄体生成素的基础水平，并进行促性腺激素释放激素刺激试验。骨龄/实际年龄比值>1且促性腺激素释放激素刺激试验中促黄体生成素峰值反应>5mIU/L的患者纳入中枢性性早熟组，不符合这些标准的患者纳入单纯性乳房早发育组。排除有器质性病变的患者。纳入年龄相仿的健康青春期前女孩作为对照组。采用酶联免疫吸附测定法测量瘦素、亲吻素和神经激肽B水平。该研究纳入了20例特发性中枢性性早熟女孩、22例单纯性乳房早发育女孩和24例青春期前对照。与对照组相比，中枢性性早熟组和单纯性乳房早发育组的血清亲吻素、瘦素和神经激肽B水平显著更高，但中枢性性早熟组和单纯性乳房早发育组之间未发现显著差异。单纯性乳房早发育和中枢性性早熟患者血清瘦素、亲吻素和神经激肽B水平升高表明它们在青春期启动过程中发挥作用，然而，这些标志物无法区分中枢性性早熟患者和单纯性乳房早发育患者。
