Noda M, Suzuki H, Numa S, Stühmer W
Department of Medical Chemistry, Kyoto University Faculty of Medicine, Japan.
FEBS Lett. 1989 Dec 18;259(1):213-6. doi: 10.1016/0014-5793(89)81531-5.
A single point mutation of the rat sodium channel II reduces its sensitivity to tetrodotoxin and saxitoxin by more than three orders of magnitude. The mutation replaces glutamic acid 387 with a glutamine and has only slight effects on the macroscopic current properties, as measured under voltage-clamp in Xenopus oocytes injected with the corresponding cDNA-derived mRNA.
大鼠钠通道II的单点突变使其对河豚毒素和石房蛤毒素的敏感性降低了三个多数量级以上。该突变将谷氨酸387替换为谷氨酰胺，并且对宏观电流特性只有轻微影响，这是在注射了相应cDNA衍生mRNA的非洲爪蟾卵母细胞中进行电压钳测量时得出的。
