供应猪乳腺的交感神经链神经节（SChG）神经元的免疫组织化学特征

Immunohistochemical Characterization of Sympathetic Chain Ganglia (SChG) Neurons Supplying the Porcine mammary Gland.

作者信息

Franke-Radowiecka A, Wąsowicz K, Klimczuk M, Podlasz P, Zalecki M, Sienkiewicz W

机构信息

Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, Olsztyn, 10-719, Poland.

出版信息

Anat Histol Embryol. 2016 Feb;45(1):44-50. doi: 10.1111/ahe.12169. Epub 2015 Jan 9.

DOI:10.1111/ahe.12169

PMID:25573117

Abstract

The aim of this study was to investigate the chemical coding of mammary gland-projecting SChG neurons using double-labelling immunohistochemistry. Earlier observation showed that after injection of the retrograde tracer fast blue (FB) into the second, right thoracic mamma, FB+ mammary gland-projecting neurons were found in Th1-3, Th9-14 and L1-4 right SChG. The greatest number of FB+ nerve cell bodies was observed in Th10 (approx. 843) and Th11 (approx. 567). Neurons projecting to the last right abdominal mamma were found in L1-4 SChG. The greatest number of FB+ neurons was observed in L2 (approx. 1200). Immunohistochemistry revealed that the vast majority of FB+ mammary-projecting neurons contained immunoreactivities to TH (96.97%) and/or DßH (95.92%). Many TH/DßH-positive neurons stained for SOM (41.5%) or NPY (33.2%), and less numerous nerve cells expressed VIP (16.9%). This observation strongly corresponds to the results of previous studies concerning the immunohistochemical characterization of nerve fibres supplying the porcine mammary gland.

摘要

本研究的目的是使用双标免疫组织化学法研究投射至乳腺的脊髓后角浅层神经元的化学编码。早期观察表明，将逆行示踪剂快蓝（FB）注射到右侧第二胸段乳腺后，在右侧脊髓后角浅层的Th1-3、Th9-14和L1-4节段发现了FB+投射至乳腺的神经元。在Th10（约843个）和Th11（约567个）观察到的FB+神经细胞体数量最多。投射至右侧最后一个腹侧乳腺的神经元在L1-4脊髓后角浅层被发现。在L2（约1200个）观察到的FB+神经元数量最多。免疫组织化学显示，绝大多数FB+投射至乳腺的神经元对TH（96.97%）和/或DßH（95.92%）呈免疫反应性。许多TH/DßH阳性神经元对SOM（41.5%）或NPY（33.2%）染色，表达VIP的神经细胞较少（16.9%）。这一观察结果与先前关于供应猪乳腺的神经纤维免疫组织化学特征的研究结果高度一致。