Song X, Hua L, Xu Y, Fang Z, Wang Y, Gao J, Shi Q, Zhou X, Yu R
Institute of Nervous System Diseases, Xuzhou Medical College, 84 West Huai-hai Road, Xuzhou, 221002, Jiangsu, People's Republic of China.
Clin Transl Oncol. 2015 Jun;17(6):477-85. doi: 10.1007/s12094-014-1263-x. Epub 2015 Jan 9.
The present study aimed at investigating the role and potential mechanism of geranylgeranyltransferase I (GGTase-I) on glioma cell migration and invasion.
Wound-healing assay and transwell assay were performed to study whether GGTase-I and Ras-like GTPase B (RalB) have effect on glioma cell migration and invasion.
We found that knockdown of GGTase-I or RalB both significantly decreased the migratory and invasive abilities of glioma cells. GGTase-I down-regulation suppressed RalB membrane association. Moreover, down-regulation of RalB partially abolished the effect of GGTβ over-expression-induced glioma cell migration and invasion increase.
These findings suggest that RalB might be one of the targets for facilitating the invasive phenotype of malignant gliomas induced by GGTase-I.
本研究旨在探讨香叶基香叶基转移酶I（GGTase-I）在胶质瘤细胞迁移和侵袭中的作用及潜在机制。
采用划痕实验和Transwell实验研究GGTase-I和类Ras GTP酶B（RalB）对胶质瘤细胞迁移和侵袭的影响。
我们发现敲低GGTase-I或RalB均显著降低胶质瘤细胞的迁移和侵袭能力。GGTase-I下调抑制RalB膜结合。此外，RalB下调部分消除了GGTβ过表达诱导的胶质瘤细胞迁移和侵袭增加的作用。
这些发现表明，RalB可能是促进GGTase-I诱导的恶性胶质瘤侵袭表型的靶点之一。
