Srinivasan Ramachandran, Kumar Velayutham Arumuga, Kumar Dileep, Ramesh Nachimuthu, Babu Subramanian, Gothandam Kodiveri Muthukalianan
School of Bio-Sciences and Technology, VIT University, Vellore, 632 014, Tamil Nadu, India.
Appl Biochem Biotechnol. 2015 Mar;175(6):2895-906. doi: 10.1007/s12010-014-1461-6. Epub 2015 Jan 10.
This study aimed to explore the effect of sodium bicarbonate (0-200 mM) on the production of β-carotene and lipid content in Dunaliella salina and Dunaliella bardawil. Total carotenoid and chlorophyll content were determined at regular intervals by a UV-VIS spectrophotometer. The β-carotene and lipid contents were analyzed using high-performance liquid chromatography (HPLC) and gas chromatography coupled with mass spectrometry (GC-MS). The HPLC results revealed a twofold increase of β-carotene in D. salina and D. bardawil cultures grown with sodium bicarbonate. Moreover, total fatty acid profiles from GC-MS indicated a maximum relative percentage of saturated fatty acids (tetradecanoic acid, 10,13-diethyl, methyl ester and methyl 16-methyl-heptadecanoate) compared to polyunsaturated fatty acids in both algae. Our results indicate that the optimum concentration of bicarbonate (100 to 150 mM) was required to stimulate a positive effect on β-carotene production as well as the lipid profile in Dunaliella sp.
本研究旨在探讨碳酸氢钠（0 - 200 mM）对盐藻和巴氏盐藻中β-胡萝卜素产量及脂质含量的影响。通过紫外可见分光光度计定期测定总类胡萝卜素和叶绿素含量。使用高效液相色谱（HPLC）以及气相色谱-质谱联用（GC-MS）分析β-胡萝卜素和脂质含量。HPLC结果显示，在添加碳酸氢钠培养的盐藻和巴氏盐藻中，β-胡萝卜素增加了两倍。此外，GC-MS的总脂肪酸谱表明，与两种藻类中的多不饱和脂肪酸相比，饱和脂肪酸（十四烷酸、10,13 - 二乙基甲酯和16 - 甲基十七烷酸甲酯）的相对百分比最高。我们的结果表明，需要最佳浓度的碳酸氢盐（100至150 mM）才能对盐藻属中β-胡萝卜素的产生以及脂质谱产生积极影响。
